Chronic Toxicity to Freshwater Organisms
40 CFR 136.3, Table I–A.This manual describes four- to seven-day methods for estimating the chronic toxicity of effluents and receiving waters to three species. The methods are approved under Clean Water Act section 304(h) and specified at
- The complete manual and individual method documents are provided. The individual methods are included in the manual.
- sheet (2016)
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Whole Effluent Toxicity Methods Errata Sheet (PDF)(28 pp, 534 K,
Corrections and clarifications to three WET methods manuals; promulgated as part of the 2017 Clean Water Act Methods Update Rule (August 28, 2017).
- Short-term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms; 4th ed. (PDF)(350 pp, 5 MB, October 2002, 821-R-02-013)
- 1000.0: Fathead Minnow, Pimephales promelas, Larval Survival and Growth; Chronic Toxicity Test (PDF)(60 pp, 1 MB, October 2002)
- 1001.0: Fathead Minnow, Pimephales promelas, Larval Survival and Teratogenicity Test; Chronic Toxicity (PDF)(30 pp, 536 K, October 2002)
- 1002.0: Daphnid, Ceriodaphnia dubia, Survival and Reproduction Test; Chronic Toxicity (PDF)(57 pp, 911 K, October 2002)
- 1003.0: Green Alga, Selenastrum capricornutum, Growth Test; Chronic Toxicity (PDF)(35 pp, 614 K, October 2002)