DRYLET™ MB BIO

TECHNICAL PRODUCT BULLETIN: B-64
USEPA, OEM REGULATIONS IMPLEMENTATION DIVISION
LISTING DATE: FEBRUARY 22, 2011
“DRYLET™ MB BIO”

I. NAME, BRAND, OR TRADEMARK
DRYLET™ MB BIO Type of Product: Bioremediation Agent (Biological Additive: Microbiological Culture) (formerly DRYLET™ MB BIOREMEDIATION)

II. NAME, ADDRESS, AND TELEPHONE NUMBER OF MANUFACTURER/CONTACT
DryLet LLC 8300 FM 1960 West Suite #450 Houston, TX 77070 Phone: (346) 980-9570 E-mail: sales@drylet.com (Mr. Scott Conley)

III. NAME, ADDRESS, AND TELEPHONE NUMBER OF PRIMARY DISTRIBUTORS
DryLet LLC 8300 FM 1960 West Suite #450 Houston, TX 77070 Phone: (346) 980-9570 E-mail: sales@drylet.com (Mr. Scott Conley)

IV. SPECIAL HANDLING AND WORKER PRECAUTIONS FOR STORAGE AND FIELD APPLICATION
1. Flammability: Non-flammable (DOT: Non-hazardous) 2. Ventilation: No special requirements. In closed spaces, uses dust protective measures. 3. Skin and eye contact; protective clothing; treatment in case of contact: No special equipment or clothing is required; however, goggles and dust mask are recommended. Applying dry powder may cause irritation to membranes. If eye or skin irritation occurs, flush with plenty of water. 4.a. Maximum storage temperature: 120°F continuous 4.b. Minimum storage temperature: 35°F 4.c. Optimum storage temperature range: 40°F to 120°F

IV. SPECIAL HANDLING AND WORKER PRECAUTIONS FOR STORAGE AND FIELD APPLICATION

1. Flammability: Non-flammable (DOT: Non-hazardous)
2. Ventilation: No special requirements. In closed spaces, uses dust protective measures.
3. Skin and eye contact; protective clothing; treatment in case of contact: No special equipment or clothing is required; however, goggles and dust mask are recommended. Applying dry powder may cause irritation to membranes. If eye or skin irritation occurs, flush with plenty of water.
4.a. Maximum storage temperature: 120°F continuous
4.b. Minimum storage temperature: 35°F
4.c. Optimum storage temperature range: 40°F to 120°F

V. SHELF LIFE
Minimum 5 years, with proper storage, in original packaging. Freezing does not harm shelf life; however, extreme heat (over 180°F) for long periods of time can shorten shelf life.

VI. RECOMMENDED APPLICATION PROCEDURE
1. Application Method: Apply DRYLET™ MB BIO directly to oil contamination. DRYLET™ MB BIO may be applied by the usual methods of aerial or manual broadcast spreading. For smaller spills, manual broadcast will deliver the best results. Larger spills in open water, marsh, wetlands, or estuary areas should be treated by aerial powder dusting or dusting with mechanical powder pumps. 2. Concentration/Application Rate: Apply DRYLET™ MB BIO directly on the spill. Apply 0.4 pounds of product per cubic yard of soil, or 50 pounds per acre of open water slick. In marsh, wetlands, and estuaries application rates may be up to 75 pounds per acre depending on the oil type and contamination level. 3. Conditions for Use: Water salinity: can be used in fresh or salt water. pH: 4 to 11.5. Temperature: 32°F – 120°F. Water Temperature: 35°F - 170°F. Nutrient Requirements: Nutrients are included with the product. However, for longer term project, additional nutrients may be added to increase microbial activity. Types and Ages of Contamination: For use on organic and hydrocarbon-based contamination. Tar like contamination may require mechanically breaking the structure to obtain timely results.

VI. RECOMMENDED APPLICATION PROCEDURE

1. Application Method: Apply DRYLET™ MB BIO directly to oil contamination. DRYLET™ MB BIO may be applied by the usual methods of aerial or manual broadcast spreading. For smaller spills, manual broadcast will deliver the best results. Larger spills in open water, marsh, wetlands, or estuary areas should be treated by aerial powder dusting or dusting with mechanical powder pumps.
2. Concentration/Application Rate: Apply DRYLET™ MB BIO directly on the spill. Apply 0.4 pounds of product per cubic yard of soil, or 50 pounds per acre of open water slick. In marsh, wetlands, and estuaries application rates may be up to 75 pounds per acre depending on the oil type and contamination level.
3. Conditions for Use: Water salinity: can be used in fresh or salt water. pH: 4 to 11.5. Temperature: 32°F – 120°F. Water Temperature: 35°F - 170°F. Nutrient Requirements: Nutrients are included with the product. However, for longer term project, additional nutrients may be added to increase microbial activity. Types and Ages of Contamination: For use on organic and hydrocarbon-based contamination. Tar like contamination may require mechanically breaking the structure to obtain timely results.

VII. TOXICITY AND EFFECTIVENESS

a. Effectiveness:
Bioremediation Agent Effectiveness Test (40 CFR 300.900), Federal Register September 15, 1994:
Summary Data Table

DAYS	PRODUCT 3 REPS/PROD	TOTAL MEAN ALKANES (ppm)	RED% 28 DAYS	TOTAL MEAN AROMATICS (ppm)	RED% 28 DAYS
0	CONTROL NUTRIENT PRODUCT	60871 61995 61459	0 0 0	8729 8170 8790	0 0 0
7	CONTROL NUTRIENT PRODUCT	56197 42501 12303	0 0 0	6224 4833 4140	0 0 0
28	CONTROL NUTRIENT PRODUCT	27314 47 820	55.0 99.9 98.6	5030 533 1305	42.4 93.5 85.1

Results of Gravimetric Analysis:
Percentage (%) Decrease in Weight of Oil on Day 28

Control:
0.30%

Nutrient:
50.0%

Product:
84.8%

b. Toxicity:
NA

VIII. MICROBIOLOGICAL ANALYSIS
1. Listing of all microorganisms by species and percentages in the composition: CONFIDENTIAL 2. Optimum pH, temperature, and salinity ranges for use of the additive: pH: 6.0-8.0 Temperature: 85°F to 110°F Salinity: 0.5-4.0 3. Minimum and maximum pH, temperature, and salinity levels below or above which the effectiveness of the additive is reduced to half its optimum capacity: pH: 4.0 or 11.5 Temperature: 35°F or 180°F Salinity: 0.5 or 5.0 (above 4.5 reduces effectiveness by half) 4. Special nutrient requirements: None 5. Test results regarding the determination of the presence of the following: Salmonella: Negative Fecal coliform: Negative Shigella: Negative Staphylococcus Coagulase positive: Negative Beta hemolytic Streptococci: Negative

VIII. MICROBIOLOGICAL ANALYSIS

1. Listing of all microorganisms by species and percentages in the composition:
CONFIDENTIAL
2. Optimum pH, temperature, and salinity ranges for use of the additive:
pH: 6.0-8.0
Temperature: 85°F to 110°F
Salinity: 0.5-4.0
3. Minimum and maximum pH, temperature, and salinity levels below or above which the effectiveness of the additive is reduced to half its optimum capacity:
pH: 4.0 or 11.5
Temperature: 35°F or 180°F
Salinity: 0.5 or 5.0 (above 4.5 reduces effectiveness by half)
4. Special nutrient requirements: None
5. Test results regarding the determination of the presence of the following:
Salmonella: Negative
Fecal coliform: Negative
Shigella: Negative
Staphylococcus Coagulase positive: Negative
Beta hemolytic Streptococci: Negative

IX. PHYSICAL PROPERTIES
NA

X. ANALYSIS FOR HEAVY METALS, CYANIDE, AND CHLORINATED HYDROCARBONS
NA

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