MICRO-BLAZE®

TECHNICAL PRODUCT BULLETIN #B-41
USEPA, OEM REGULATIONS IMPLEMENTATION DIVISION
ORIGINAL LISTING DATE: DECEMBER 18,1991
REVISED LISTING DATE: JANUARY 21, 1997
"MICRO-BLAZE®"

I. NAME, BRAND, OR TRADEMARK
MICRO-BLAZE® Type of Product: Biological Additive

II. NAME, ADDRESS, AND TELEPHONE NUMBER OF MANUFACTURER/CONTACT
Verde Environmental, Inc. 9223 Eastex Freeway Houston, TX 77093 Phone: (713) 691-6468 Phone: (800) 626-6598 Fax: (713) 691-2331 Website: http://www.micro-blaze.com/site/ Exit Email: bscogin@micro-blaze.com (Mr. William Scogin)

III. NAME, ADDRESS, AND TELEPHONE NUMBER OF PRIMARY DISTRIBUTORS
Verde Environmental, Inc. 9223 Eastex Freeway Houston, TX 77093 Phone: (713) 691-6468 or Phone: (800) 626-6598 Fax: (713) 691-2331 Website: http://www.micro-blaze.com/site/ Exit Email: bscogin@micro-blaze.com (Mr. William Scogin)

IV. SPECIAL HANDLING AND WORKER PRECAUTIONS FOR STORAGE AND FIELD APPLICATION
1. Flammability: Non-flammable 2. Ventilation: Normal room ventilation 3. Skin and eye contact; protective clothing; treatment in case of contact: Avoid eye contact. Wear protective gloves, and wash hands with soap and water after handling the product. Wash contaminated clothing and footwear before reuse. 4.a. Maximum storage temperature: 120ºF 4.b. Minimum storage temperature: 35ºF 4.c. Optimum storage temperature range: NA 4.d. Temperatures of phase separations and chemical changes: NA

IV. SPECIAL HANDLING AND WORKER PRECAUTIONS FOR STORAGE AND FIELD APPLICATION

1. Flammability:
Non-flammable
2. Ventilation:
Normal room ventilation
3. Skin and eye contact; protective clothing; treatment in case of contact:
Avoid eye contact. Wear protective gloves, and wash hands with soap and water after handling the product. Wash contaminated clothing and footwear before reuse.
4.a. Maximum storage temperature: 120ºF
4.b. Minimum storage temperature: 35ºF
4.c. Optimum storage temperature range: NA
4.d. Temperatures of phase separations and chemical changes: NA

V. SHELF LIFE
Minimum 10 years, with proper storage, in original container. Freezing will not kill these microbes; however, extreme heat for long periods of time (over 180ºF) will kill the microbes.

VI. RECOMMENDED APPLICATION PROCEDURE
MICRO-BLAZE® is a liquid formulation of several microbial strains, surfactants and nutrients designed to digest organics and hydrocarbons in soil and water as well as control odors. 1. Application Method: Use normal spray equipment, fire or response equipment, eductor setups, water trucks, etc. as methods of application. Mix MICRO-BLAZE®/water mixture with contaminated soils and liquids throughly for maximum contact. Pick up treated contamination after volatile hazard has been negated per local regulatory parameters. For general bioremediation: For in-situ soils, mix MICRO-BLAZE®/water mixture can be tilled into the contaminated area. For shallower contamination, areas can be oversprayed with normal spray equipment, eductor setups, water trucks, etc. For deeper contamination, application can be applied through underground setups using perforated piping per regulatory recommendations. For wastewater and other operational by-product sludges and soils extracted from contaminated areas, a bioslurry or bioreactor can be setup using MICRO-BLAZE® and water in the process. 2. Concentration/Application Rate: For more viscous or less hazardous contamination, apply MICRO-BLAZE® at a 3% solution mixed with water (3 parts MICRO-BLAZE®, 97 parts water). When bioremediating soils, generally, per every 10 cubic yards of contaminated soils, use one gallon of concentrate, diluted with water according to contamination type. 3. Conditions for Use: Water Salinity: Can be mixed with any fresh, brackish or brine. However, brine reduces the effectiveness by 10%. Water Temperature: 35ºF - 180ºF pH: 4 to 11.5 Temperature: 32ºF - 120ºF Nutrient Requirements: Nutrients for microbes are included in product. However, for longer-term bioremediation projects, additional applications for Bio-Catalyst may be added to boost microbial activity. Type and Ages of Pollutants: For use on organics and hydrocarbon-based materials. These strains of bacteria provide the capability of biodegrading various straight chained, branched chained, aromatic and polynuclear aromatic hydrocarbons found in diesel and other fuels. Age of contamination is not a factor as much as its density. Tar-like substances may need to be cut for timely remediation.

VI. RECOMMENDED APPLICATION PROCEDURE

MICRO-BLAZE® is a liquid formulation of several microbial strains, surfactants and nutrients designed to digest organics and hydrocarbons in soil and water as well as control odors.
1. Application Method:
Use normal spray equipment, fire or response equipment, eductor setups, water trucks, etc. as methods of application. Mix MICRO-BLAZE®/water mixture with contaminated soils and liquids throughly for maximum contact. Pick up treated contamination after volatile hazard has been negated per local regulatory parameters. For general bioremediation: For in-situ soils, mix MICRO-BLAZE®/water mixture can be tilled into the contaminated area. For shallower contamination, areas can be oversprayed with normal spray equipment, eductor setups, water trucks, etc. For deeper contamination, application can be applied through underground setups using perforated piping per regulatory recommendations. For wastewater and other operational by-product sludges and soils extracted from contaminated areas, a bioslurry or bioreactor can be setup using MICRO-BLAZE® and water in the process.
2. Concentration/Application Rate:
For more viscous or less hazardous contamination, apply MICRO-BLAZE® at a 3% solution mixed with water (3 parts MICRO-BLAZE®, 97 parts water). When bioremediating soils, generally, per every 10 cubic yards of contaminated soils, use one gallon of concentrate, diluted with water according to contamination type.
3. Conditions for Use:
Water Salinity: Can be mixed with any fresh, brackish or brine. However, brine reduces the effectiveness by 10%.
Water Temperature: 35ºF - 180ºF
pH: 4 to 11.5
Temperature: 32ºF - 120ºF
Nutrient Requirements: Nutrients for microbes are included in product. However, for longer-term bioremediation projects, additional applications for Bio-Catalyst may be added to boost microbial activity.
Type and Ages of Pollutants: For use on organics and hydrocarbon-based materials. These strains of bacteria provide the capability of biodegrading various straight chained, branched chained, aromatic and polynuclear aromatic hydrocarbons found in diesel and other fuels. Age of contamination is not a factor as much as its density. Tar-like substances may need to be cut for timely remediation.

VII. TOXICITY AND EFFECTIVENESS

Non-toxic, naturally-occurring spore-forming microorganisms common to soil and water. Non-pathogenic, certified by count; will not mutate.

a. Toxicity
NA

b. Effectiveness
Bioremediation Agent Effectiveness Test (40 CFR 300,900) Federal Register September 15, 1994.
Microbiological Results - Average
Day 0 - 1.7 x 109
Day 7 - 8.43 x 108
Day 28 - 5.2 x 107
The organisms in this product convert to a spore state (dormant) to survive an unfavorable environment and will reactivate upon favorable conditions. Documentation available from Verde Environmental.

Summary Data Table:

DAYS	PRODUCT 3 REPS/PROD	TOTAL MEAN ALKANES (ppm)	RED% 28 Days	TOTAL MEAN AROMATICS (ppm)	RED% 28 DAYS
0	CONTROL NUTRIENT MICRO-BLAZE®	31258.6 28251.8 29548.9	0 0 0	973 976.6 1081.2	0 0 0
7	CONTROL NUTRIENT MICRO-BLAZE®	31401.73 20728.3 12870.5	0 26.6 56.4	990.5 619.1 496.3	0 36.6 54.1
28	CONTROL NUTRIENT MICRO-BLAZE®	32465.8 1787.2 1758.2	0 93.7 94.1	925.7 722.6 566.9	0 26.0 47.6

Alkanes showed significant reductions with aromatic components less dramatic but still significant.

Results of Gravimetric Analysis:
Percentage (%) Decrease in Weight of Oil on Day 28
CONTROL: < 1%
NUTRIENT: 17.6%
MICRO-BLAZE®: 12%
CONCLUSIONS: The MICRO-BLAZE® product shows an initial rapid consumption of all measured hydrocarbons at seven (7) days. This rate apparently slows over 28-day period in a closed environment which may be due to a change in the environment of the flask due to the rapid degradation rates. Because of the high microbial population at the end of the test, it is to be assumed that the quantity of metabolites might account for the increased weight as determined by the gravimetric analysis.

VIII. MICROBIOLOGICAL ANALYSIS
1. Listing of all micro-organisms by species and percentage in the composition: CONFIDENTIAL 2. Optimum pH, temperature, and salinity ranges for use of the additive: pH 7.5 Temperature: 45ºF - 105ºF Salinity: 0 - 10% 3. Minimum and maximum pH, temperature, and salinity levels below or above which the effectiveness of the additive is reduced to half its optimum capacity: pH: <5.9, >9.0 Temperature: <32ºF, >180ºF Salinity: <0%, 10% 4. Special nutrient requirements: none 5. Test results regarding the determination of the presence of the following: Salmonella: Negative Fecal coliform: Negative Shigella: Negative Staphylococcus Coagulase positive: Negative Beta hemolytic Streptococci: Negative

VIII. MICROBIOLOGICAL ANALYSIS

1. Listing of all micro-organisms by species and percentage in the composition: CONFIDENTIAL
2. Optimum pH, temperature, and salinity ranges for use of the additive:
pH 7.5
Temperature: 45ºF - 105ºF
Salinity: 0 - 10%
3. Minimum and maximum pH, temperature, and salinity levels below or above which the effectiveness of the additive is reduced to half its optimum capacity:
pH: <5.9, >9.0
Temperature: <32ºF, >180ºF
Salinity: <0%, 10%
4. Special nutrient requirements: none
5. Test results regarding the determination of the presence of the following:
Salmonella: Negative
Fecal coliform: Negative
Shigella: Negative
Staphylococcus Coagulase positive: Negative
Beta hemolytic Streptococci: Negative

IX. PHYSICAL PROPERTIES
NA

X. ANALYSIS OF HEAVY METALS, CYANIDE, AND CHLORINATED HYDROCARBONS
NA

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