OPPENHEIMER FORMULA

TECHNICAL PRODUCT BULLETIN #B-36
USEPA, OEM REGULATIONS IMPLEMENTATION DIVISION
ORIGINAL LISTING DATE: JULY 17, 1991
REVISED LISTING DATE: OCTOBER 6, 1996
"OPPENHEIMER FORMULA"
(a/k/a The OPPENHEIMER FORMULA I, MICROSORB SC)

I. NAME, BRAND, OR TRADEMARK
OPPENHEIMER FORMULA (a/k/a The OPPENHEIMER FORMULA I, MICROSORB SC) Type of Product: Biological Additive

II. NAME, ADDRESS, AND TELEPHONE NUMBER OF MANUFACTURER/CONTACT
Oppenheimer Biotechnology, Inc. P.O. Box 1490 Pflugerville, TX 78691-1490 Phone: (512) 474-1016 Fax: (512) 681-0367 Email: jen.neve@obio.com Web Site: www.obio.com Exit (Ms. Jen Neve)

III. NAME, ADDRESS, AND TELEPHONE NUMBER OF PRIMARY DISTRIBUTORS
Oppenheimer Biotechnology, Inc. P.O. Box 1490 Pflugerville, TX 78691-1490 Phone: (512) 474-1016 Fax: (512) 681-0367 Email: jen.neve@obio.com Web Site: www.obio.com Exit (Ms. Jen Neve)

IV. SPECIAL HANDLING AND WORKER PRECAUTIONS FOR STORAGE AND FIELD APPLICATION
1. Flammability: Non-flammable 2. Ventilation: Special ventilation is not required. Treat product as an hygroscopic powder. In closed spaces use dust protective measures. 3. Skin and eye contact; protective clothing; treatment in case of contact: The application of the dry powder requires the usual precautions of a dust irritant to membranes. The material is easily removed by washing or flushing, and in confined areas or use, a protective mask and eye glasses are recommended. 4.a. Maximum storage temperature: 130ºF 4.b. Minimum storage temperature: 32ºF 4.c. Optimum storage temperature range: 82ºF

IV. SPECIAL HANDLING AND WORKER PRECAUTIONS FOR STORAGE AND FIELD APPLICATION

1. Flammability:
Non-flammable
2. Ventilation:
Special ventilation is not required. Treat product as an hygroscopic powder. In closed spaces use dust protective measures.
3. Skin and eye contact; protective clothing; treatment in case of contact:
The application of the dry powder requires the usual precautions of a dust irritant to membranes. The material is easily removed by washing or flushing, and in confined areas or use, a protective mask and eye glasses are recommended.
4.a. Maximum storage temperature: 130ºF
4.b. Minimum storage temperature: 32ºF
4.c. Optimum storage temperature range: 82ºF

V. SHELF LIFE
The shelf life of the product is approximately 5 year.

VI. RECOMMENDED APPLICATION PROCEDURE
1. Application Method: Optimal application is by powder seeding directly at the source of oil contamination. Application over larger areas of water surface can be accomplished by aerial powder dusting, spraying a water mixture of nutrients/formula over the oil area, or by dusting with mechanical powder pumps. 2. Concentration/Application Rate: The application rate at the source oil slick is 1 pound per cubic yard of oil. In the open sea slick, the basic application rate is 10 pounds per acre, but this may vary in different situations related to speed of clean up. In estuaries, a larger biomass is recommended and may be at a rate of up to 100 pounds per acre, depending on the oil type and concentration. Heavier oils may require a higher biomass than light oils. 3. Conditions for Use: THE OPPENHEIMER FORMULA may be used in a wide range of environmental conditions of temperature and salinities, ranging from fresh to 100 ppt salts. The formula is especially valuable in grass flats, marshes and the open sea. It has also proved effective in soil and fresh water oil clean up and municipal waste treatment, septic tanks and grease traps.

VI. RECOMMENDED APPLICATION PROCEDURE

1. Application Method:
Optimal application is by powder seeding directly at the source of oil contamination. Application over larger areas of water surface can be accomplished by aerial powder dusting, spraying a water mixture of nutrients/formula over the oil area, or by dusting with mechanical powder pumps.
2. Concentration/Application Rate:
The application rate at the source oil slick is 1 pound per cubic yard of oil. In the open sea slick, the basic application rate is 10 pounds per acre, but this may vary in different situations related to speed of clean up. In estuaries, a larger biomass is recommended and may be at a rate of up to 100 pounds per acre, depending on the oil type and concentration. Heavier oils may require a higher biomass than light oils.
3. Conditions for Use:
THE OPPENHEIMER FORMULA may be used in a wide range of environmental conditions of temperature and salinities, ranging from fresh to 100 ppt salts. The formula is especially valuable in grass flats, marshes and the open sea. It has also proved effective in soil and fresh water oil clean up and municipal waste treatment, septic tanks and grease traps.

VII. TOXICITY AND EFFECTIVENESS

a. Toxicity:
NA

b. Effectiveness:
Bioremediation Agent Effectiveness Test (40 CFR 300.900), Federal Register September 15, 1994:

Summary Data Table:

DAYS	PRODUCT 3 REPS/PROD	TOTAL MEAN ALKANES (ppm)	RED% 28 DAYS	TOTAL MEAN AROMATICS (ppm)	RED% 28 DAYS
0	CONTROL NUTRIENT OPPENHEIMER	29965.2 31494.8 30101.5	0 0 0	5620.5 6660.2 5528.2	0 0 0
7	CONTROL NUTRIENT OPPENHEIMER	30101.5 8073.9 5019.7	0 0 0	5610.1 5086.6 4338.9	0 0 0
28	CONTROL NUTRIENT OPPENHEIMER	28785.6 706.2 3396.8	3.9 97.8 89.1	5512.7 4863.7 3417.8	1.9 27.0 38.2

Results of Gravimetric Analysis:
Percentage (%) Decrease in Weight of Oil on Day 28
Control: - 6.4 %
Nutrient: 50.9 %
Product (Oppenheimer): 10.4 %

VIII. MICROBIOLOGICAL ANALYSIS
1. Listing of all microorganisms by species and percentage in the composition: Natural, ubiquitous, hydrocarbon-oxidizing, microorganisms for use in removing hydrocarbons and organic materials from soils, fresh and salt waters by natural oxidative pathways. 2. Optimum pH, temperature, and salinity ranges for use of the additive: pH: 7.6 Temperature: 82ºF Salinity: Fresh water to 20 percent salts. Optimal: 0.5 to 3.5 percent. 3. Minimum and maximum pH, temperature, and salinity levels below or above which the effectiveness of the additive is reduced to half its optimum capacity: pH: 5.5-11 Temperature: 32ºF-140ºF Salinity: Fresh to 15% 4. Special nutrient requirements: NA 5. Test results regarding the determination of the presence of the following: Salmonella: Negative Fecal Streptococci: < 90/100 ml Shigella: Negative Staphylococcus Coagulase positive: Negative Beta hemolytic Streptococci: Negative

VIII. MICROBIOLOGICAL ANALYSIS

1. Listing of all microorganisms by species and percentage in the composition:
Natural, ubiquitous, hydrocarbon-oxidizing, microorganisms for use in removing hydrocarbons and organic materials from soils, fresh and salt waters by natural oxidative pathways.
2. Optimum pH, temperature, and salinity ranges for use of the additive:
pH: 7.6
Temperature: 82ºF
Salinity: Fresh water to 20 percent salts.
Optimal: 0.5 to 3.5 percent.
3. Minimum and maximum pH, temperature, and salinity levels below or above which the effectiveness of the additive is reduced to half its optimum capacity:
pH: 5.5-11
Temperature: 32ºF-140ºF
Salinity: Fresh to 15%
4. Special nutrient requirements:
NA
5. Test results regarding the determination of the presence of the following:
Salmonella: Negative
Fecal Streptococci: < 90/100 ml
Shigella: Negative
Staphylococcus Coagulase positive: Negative
Beta hemolytic Streptococci: Negative

IX. PHYSICAL PROPERTIES
NA

X. ANALYSIS OF HEAVY METALS, CYANIDE, AND CHLORINATED HYDROCARBONS
NA

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