PETROCLEAN™

TECHNICAL PRODUCT BULLETIN: B-72
USEPA, OEM REGULATIONS IMPLEMENTATION DIVISION
LISTING DATE: APRIL 2, 2018
“PETROCLEAN™”

I. NAME, BRAND, OR TRADEMARK
PETROCLEAN™ Type of Product: Bioremediation Agent (Biological Additive/Microbiological Culture)

II. NAME, ADDRESS, AND TELEPHONE NUMBER OF MANUFACTURER/CONTACT
Green Earth Naturally, L.L.C. 2314 Ridgefield Street NE Roanoke, VA 24102 Phone: (540) 362-5636 Fax: (540) 362-9447 E-mail: bcarroll@greenearthnaturally.com Website: www.GreenEarthNaturally.com Exit (Mr. Brian Carroll)

III. NAME, ADDRESS, AND TELEPHONE NUMBER OF PRIMARY DISTRIBUTORS
Green Earth Naturally, L.L.C. 2314 Ridgefield Street NE Roanoke, VA 24102 Phone: (540) 362-5636 Fax: (540) 362-9447 E-mail: bcarroll@greenearthnaturally.com Website: www.GreenEarthNaturally.com Exit (Mr. Brian Carroll)

IV. SPECIAL HANDLING AND WORKER PRECAUTIONS FOR STORAGE AND FIELD APPLICATION
1. Flammability: Non-flammable (DOT: Non-hazardous) 2. Ventilation: Use combination of general dilution ventilation and local exhaust ventilation to keep exposure as low as possible. 3. Skin and eye contact; protective clothing; treatment in case of contact: Prevent skin and eye contact by wearing appropriate personal protective equipment. If eye or skin irritation occurs, flush with plenty of fresh water. Coveralls or other suitable protective garments should be worn with suitable footwear for application and working conditions. Chemical safety goggles or glasses and impervious gloves are recommended. 4.a. Maximum storage temperature: below 120ºF 4.b. Minimum storage temperature: above freezing 4.c. Optimum storage temperature range: 55ºF to 100ºF. Avoid storage in areas of extreme heat or extreme cold. Store in tightly closed containers, and away from children. 4.d. Temperatures of phase separation and chemical changes: Stable

IV. SPECIAL HANDLING AND WORKER PRECAUTIONS FOR STORAGE AND FIELD APPLICATION

1. Flammability: Non-flammable (DOT: Non-hazardous)
2. Ventilation: Use combination of general dilution ventilation and local exhaust ventilation to keep exposure as low as possible.
3. Skin and eye contact; protective clothing; treatment in case of contact: Prevent skin and eye contact by wearing appropriate personal protective equipment. If eye or skin irritation occurs, flush with plenty of fresh water. Coveralls or other suitable protective garments should be worn with suitable footwear for application and working conditions. Chemical safety goggles or glasses and impervious gloves are recommended.
4.a. Maximum storage temperature: below 120ºF
4.b. Minimum storage temperature: above freezing
4.c. Optimum storage temperature range: 55ºF to 100ºF. Avoid storage in areas of extreme heat or extreme cold. Store in tightly closed containers, and away from children.
4.d. Temperatures of phase separation and chemical changes: Stable

V. SHELF LIFE
Three to six months in sealed 1gallon containers, 5 gallon buckets and 55 gallon drums or totes (as delivered).

VI. RECOMMENDED APPLICATION PROCEDURE
PETROCLEAN™ is effective in bioremediating petroleum contaminated soils and is not designed for use in open waters. There are no limitations to usage within optimum temperature parameters. Application Method: Surficial spraying of PETROCLEAN™ may be applied by any type of liquid delivery system. Soils and gravels can be treated with PETROCLEAN™ using a conventional yard sprayer. Repetition may be needed. PETROCLEAN™ can be applied to secondary containment areas, the key factor in cleaning the contaminated substrate, whether gravel or soil, is residence time for the microbes, moisture, and oxygen. Subsurface application can vary by location. Soil type becomes a contingent factor in dilution rates and method of application. When bioremediating soils, cleaning the contaminated substrate, whether gravel or soil, is affected by residence time for the microbes, moisture, and oxygen. For in-situ soils, mix PETROCLEAN™ with water to the desired concentration and dispense inoculated fluids in the subsurface, adjacent infrastructure, and the surface. For ex-situ soils and longer term bioremediation projects, additional applications of nutrients and oxygen releasing compounds may be required to maintain or boost microbial activity. Concentration/Application Rate: Rates will vary based upon the type of soil and amount of petroleum spilled, the extent of weathering, and other site-specific conditions, including soil profiles, proximity to surface waters and wells. Heavy petroleum compounds such as crude oil and bunker fuel, with a higher proportion of complex aliphatics, will require a higher concentration of PETROCLEAN™ than lighter petroleum compounds such as gasoline. Hydrocarbon types: PETROCLEAN™ can be applied on any hydrocarbon compound (including crude, diesel, gasoline, aviation fuel, bunker fuels, etc.) found in soils. Most frequent applications include any petroleum contamination around AST’s and USTs and containment pits requiring rehabilitation. Concentration Rate: on heavy oils, apply PETROCLEAN™ directly on the spill area following absorption of free product, utilize a 1:10 dilution ratio (product to water). Lighter oils will require a 1:30 (product to water) dilution ratio. Conditions for Use: Bioremediation slows at temperatures below 40ºF. No limitations as to usage within optimum temperature parameters have been observed. Monitoring of dissolved oxygen levels is recommended. If needed, a mechanical aeration device should be deployed or the use of Oxygen Releasing Compounds (ORC) should be encouraged to maintain dissolved oxygen levels above 3 mg/L. Additional nutrients may be required to increase microbial activity over time if intrinsic nutrients are depleted. The application of 10% nitrogen, 10% phosphorus, and 10% potassium fertilizer can be used in-situ or ex-situ as situations warrant to boost microbial activity and enhance co-metabolic digestion of petroleum. Prevent spill run-off from entering surface water bodies. Eliminate all source of ignition. Dispose of used absorbent materials according to all local, regional, and federal regulations.

VI. RECOMMENDED APPLICATION PROCEDURE

PETROCLEAN™ is effective in bioremediating petroleum contaminated soils and is not designed for use in open waters. There are no limitations to usage within optimum temperature parameters.

Application Method:
1. Surficial spraying of PETROCLEAN™ may be applied by any type of liquid delivery system.
  1. Soils and gravels can be treated with PETROCLEAN™ using a conventional yard sprayer. Repetition may be needed.
  2. PETROCLEAN™ can be applied to secondary containment areas, the key factor in cleaning the contaminated substrate, whether gravel or soil, is residence time for the microbes, moisture, and oxygen.
2. Subsurface application can vary by location. Soil type becomes a contingent factor in dilution rates and method of application. When bioremediating soils, cleaning the contaminated substrate, whether gravel or soil, is affected by residence time for the microbes, moisture, and oxygen.
  1. For in-situ soils, mix PETROCLEAN™ with water to the desired concentration and dispense inoculated fluids in the subsurface, adjacent infrastructure, and the surface.
  2. For ex-situ soils and longer term bioremediation projects, additional applications of nutrients and oxygen releasing compounds may be required to maintain or boost microbial activity.
Concentration/Application Rate: Rates will vary based upon the type of soil and amount of petroleum spilled, the extent of weathering, and other site-specific conditions, including soil profiles, proximity to surface waters and wells. Heavy petroleum compounds such as crude oil and bunker fuel, with a higher proportion of complex aliphatics, will require a higher concentration of PETROCLEAN™ than lighter petroleum compounds such as gasoline.
1. Hydrocarbon types: PETROCLEAN™ can be applied on any hydrocarbon compound (including crude, diesel, gasoline, aviation fuel, bunker fuels, etc.) found in soils.
2. Most frequent applications include any petroleum contamination around AST’s and USTs and containment pits requiring rehabilitation.
3. Concentration Rate: on heavy oils, apply PETROCLEAN™ directly on the spill area following absorption of free product, utilize a 1:10 dilution ratio (product to water). Lighter oils will require a 1:30 (product to water) dilution ratio.
Conditions for Use: Bioremediation slows at temperatures below 40ºF. No limitations as to usage within optimum temperature parameters have been observed.
1. Monitoring of dissolved oxygen levels is recommended. If needed, a mechanical aeration device should be deployed or the use of Oxygen Releasing Compounds (ORC) should be encouraged to maintain dissolved oxygen levels above 3 mg/L.
2. Additional nutrients may be required to increase microbial activity over time if intrinsic nutrients are depleted. The application of 10% nitrogen, 10% phosphorus, and 10% potassium fertilizer can be used in-situ or ex-situ as situations warrant to boost microbial activity and enhance co-metabolic digestion of petroleum.
3. Prevent spill run-off from entering surface water bodies.
4. Eliminate all source of ignition.
5. Dispose of used absorbent materials according to all local, regional, and federal regulations.

VII.TOXICITY AND EFFECTIVENESS

Effectiveness:
Bioremediation Agent Effectiveness Test (40 CFR 300.900), Federal Register September 15, 1994:

Summary Data Table

DAYS	ACT Terra Firma 3 REPS/PROD	TOTAL MEAN ALKANES (ppm)	RED% 28 DAYS	TOTAL MEAN AROMATICS (ppm)	RED% 28 DAYS
0	CONTROL	57,032	0	12,547	0
	NUTRIENT	55,231	0	12,547	0
	PETROCLEAN™	58,148	0	12,141	0
7	CONTROL	55,126	0	12,091	0
	NUTRIENT	47,951	0	11,599	0
	PETROCLEAN™	34,095	0	8,640	0
28	CONTROL	50,608	11.2%	10,766	14.2%
	NUTRIENT	34,291	37.9%	9,574	23.1%
	PETROCLEAN™	14,854	74.4%	4,711	61.1%

Results of Gravimetric Analysis:

Percentage (%) Decrease in Weight of Oil on Day 28

Control	Nutrient	PETROCLEAN™
7.83%	41.50%	60.93%

b. Toxicity:

VIII. MICROBIOLOGICAL ANALYSIS
1. Listing of each component of the total formulation, other than microorganisms, by chemical name and percentage by weight: CONFIDENTIAL 2. Listing of all microorganisms by species and percentages of each species in the composition of the additive: CONFIDENTIAL 3. Optimum pH, temperature, and salinity ranges for use of the additive: pH: range 6.3 to 7.3 Temperature: range 55°F to 100°F Salinity: 0 to 5% 3. Minimum and maximum pH, temperature, and salinity levels below or above which the effectiveness of the additive is reduced to half its optimum capacity: pH: 6.0 and 8.0 Temperature: >32°F and <120°F Salinity: no minimum and 10% 4. Special nutrient requirements: NA 5. Test results regarding the determination of the presence of the following: Salmonella: Negative Fecal coliform: Negative Shigella: Negative Staphylococcus Coagulase positive: Negative Beta hemolytic Streptococci: Negative

VIII. MICROBIOLOGICAL ANALYSIS

1. Listing of each component of the total formulation, other than microorganisms, by chemical name and percentage by weight: CONFIDENTIAL
2. Listing of all microorganisms by species and percentages of each species in the composition of the additive: CONFIDENTIAL
3. Optimum pH, temperature, and salinity ranges for use of the additive:
pH: range 6.3 to 7.3
Temperature: range 55°F to 100°F
Salinity: 0 to 5%
3. Minimum and maximum pH, temperature, and salinity levels below or above which the effectiveness of the additive is reduced to half its optimum capacity:
pH: 6.0 and 8.0
Temperature: >32°F and <120°F
Salinity: no minimum and 10%
4. Special nutrient requirements: NA
5. Test results regarding the determination of the presence of the following:
Salmonella: Negative
Fecal coliform: Negative
Shigella: Negative
Staphylococcus Coagulase positive: Negative
Beta hemolytic Streptococci: Negative

IX. PHYSICAL PROPERTIES
NA

X. ANALYSIS FOR HEAVY METALS, CYANIDE, AND CHLORINATED HYDROCARBONS
NA

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