PRO-ACT

TECHNICAL PRODUCT BULLETIN: B-62
USEPA, OEM REGULATIONS IMPLEMENTATION DIVISION
LISTING DATE: DECEMBER 15, 2010
“PRO-ACT”

I. NAME, BRAND, OR TRADEMARK
PRO-ACT (aka, OILCLEAN w/ACTIVATOR) Type of Product: Bioremediation Agent (Biological Additive: Microbiological Culture w/ Nutrient Additive)

II. NAME, ADDRESS, AND TELEPHONE NUMBER OF MANUFACTURER/CONTACT
Pro-Act Biotech a Unit of LLG.LLC 64 Church Street Warren, RI 02885 Phone: (401) 245-7004 Fax: (401) 633-6270 E-mail: bill@proactbiotech.com Web Site: www.oil-clean.net Exit (Mr. William Campion)

III. NAME, ADDRESS, AND TELEPHONE NUMBER OF PRIMARY DISTRIBUTORS
Pro-Act Biotech a Unit of LLG.LLC 64 Church Street Warren, RI 02885 Phone: (401) 245-7004 Fax: (401) 633-6270 E-mail: bill@proactbiotech.com Web Site: www.oil-clean.net Exit (Mr. William Campion)

IV. SPECIAL HANDLING AND WORKER PRECAUTIONS FOR STORAGE AND FIELD APPLICATION
1. Flammability: Non-flammable 2. Ventilation: No special requirements. 3. Skin and eye contact; protective clothing; treatment in case of contact: No special equipment or clothing is required in the handling, storage and field application of this product. For skin and eye contact, wear goggles. If eye or skin irritation occurs, flush with plenty of fresh water. 4.a. Maximum storage temperature: 110°F, up to 5 days 4.b. Minimum storage temperature: 35°F, do not freeze 4.c. Optimum storage temperature range: 40°F to 90°F

IV. SPECIAL HANDLING AND WORKER PRECAUTIONS FOR STORAGE AND FIELD APPLICATION

1. Flammability: Non-flammable
2. Ventilation: No special requirements.
3. Skin and eye contact; protective clothing; treatment in case of contact: No special equipment or clothing is required in the handling, storage and field application of this product. For skin and eye contact, wear goggles. If eye or skin irritation occurs, flush with plenty of fresh water.
4.a. Maximum storage temperature: 110°F, up to 5 days
4.b. Minimum storage temperature: 35°F, do not freeze
4.c. Optimum storage temperature range: 40°F to 90°F

V. SHELF LIFE
One year as packaged.

VI. RECOMMENDED APPLICATION PROCEDURE
PRO-ACT (aka, OILCLEAN w/ACTIVATOR) consists of two parts: liquid microbes and activator (i.e., nutrient additive). As part of the Product Schedule, training on the proper use of PRO-ACT (aka, OILCLEAN w/ACTIVATOR) will be provided by Pro-Act Microbial, Inc. certified personnel. 1. Application Method: Site (water and/or soil) testing is highly recommended prior to product application. The testing is designed to assess the nutrient status of the contaminated site. The desired nitrogen and phosphorus concentrations are in the range of 0.5 to 2.5 mg N/L for nitrogen and 0.05 to 0.3 mg P/L for phosphorus. If there are inadequate nutrients in the water/soil, the activator portion will be needed to bring the nutrients in line with product specification. The activator portion can be applied directly to the site for treatment or it can be mixed in with the diluted liquid microbes first. Nutrient levels need to be maintained for optimal microbial action. Thus, depending on the nature of water/soil and the results of on site monitoring, activator may need to be applied more than once. It is recommended that physical methods such as skimming should be employed to recover as much oil as possible. PRO-ACT (aka, OILCLEAN w/ACTIVATOR) should be used to breakdown the residual oil after the physical methods have been used. 2. Concentration/Application Rate: Liquid microbes are to be diluted with the non-chlorinated, non-ozonated water or with the water to be treated (1:10 liquid microbe/water ratio) and applied with a sprayer, either pump or pneumatic. The amount of PRO-ACT (aka, OILCLEAN w/ACTIVATOR) needed depends on the amount of oil to be treated. One gallon undiluted liquid microbes can treat up to 1000 square feet, assuming there is less than ¼ inch of oil on surface. For example, one gallon undiluted liquid microbes is good for up to 5 gallons of spilled oil. A second application of Pro-Act (aka OilClean w/Activator) is beneficial to speed up the bioremediation process. 3. Conditions for Use: Monitoring of dissolved oxygen levels is recommended. If needed, a mechanical aeration device should be deployed to maintain DO levels above 3 mg/L. PRO-ACT (aka, OILCLEAN w/ACTIVATOR) works with fresh oil as well as weathered oil. Oil toxicity (especially from easily dissolved shorter chain hydrocarbons in the fresh oil) should not be a problem as long as physical methods are used first following a spill. The product is ideal for salt water, brackish water and fresh water, with no limitations as to usage within optimum temperature range of 20 to 400°C.

VI. RECOMMENDED APPLICATION PROCEDURE

PRO-ACT (aka, OILCLEAN w/ACTIVATOR) consists of two parts: liquid microbes and activator (i.e., nutrient additive). As part of the Product Schedule, training on the proper use of PRO-ACT (aka, OILCLEAN w/ACTIVATOR) will be provided by Pro-Act Microbial, Inc. certified personnel.
1. Application Method: Site (water and/or soil) testing is highly recommended prior to product application. The testing is designed to assess the nutrient status of the contaminated site. The desired nitrogen and phosphorus concentrations are in the range of 0.5 to 2.5 mg N/L for nitrogen and 0.05 to 0.3 mg P/L for phosphorus. If there are inadequate nutrients in the water/soil, the activator portion will be needed to bring the nutrients in line with product specification. The activator portion can be applied directly to the site for treatment or it can be mixed in with the diluted liquid microbes first. Nutrient levels need to be maintained for optimal microbial action. Thus, depending on the nature of water/soil and the results of on site monitoring, activator may need to be applied more than once. It is recommended that physical methods such as skimming should be employed to recover as much oil as possible. PRO-ACT (aka, OILCLEAN w/ACTIVATOR) should be used to breakdown the residual oil after the physical methods have been used.
2. Concentration/Application Rate: Liquid microbes are to be diluted with the non-chlorinated, non-ozonated water or with the water to be treated (1:10 liquid microbe/water ratio) and applied with a sprayer, either pump or pneumatic. The amount of PRO-ACT (aka, OILCLEAN w/ACTIVATOR) needed depends on the amount of oil to be treated. One gallon undiluted liquid microbes can treat up to 1000 square feet, assuming there is less than ¼ inch of oil on surface. For example, one gallon undiluted liquid microbes is good for up to 5 gallons of spilled oil. A second application of Pro-Act (aka OilClean w/Activator) is beneficial to speed up the bioremediation process.
3. Conditions for Use: Monitoring of dissolved oxygen levels is recommended. If needed, a mechanical aeration device should be deployed to maintain DO levels above 3 mg/L. PRO-ACT (aka, OILCLEAN w/ACTIVATOR) works with fresh oil as well as weathered oil. Oil toxicity (especially from easily dissolved shorter chain hydrocarbons in the fresh oil) should not be a problem as long as physical methods are used first following a spill. The product is ideal for salt water, brackish water and fresh water, with no limitations as to usage within optimum temperature range of 20 to 400°C.

VII. TOXICITY AND EFFECTIVENESS

a. Effectiveness:
Bioremediation Agent Effectiveness Test (40 CFR 300.900), Federal Register September 15, 1994:
Summary Data Table

DAYS	PRODUCT 3 REPS/PROD	TOTAL MEAN ALKANES (ppm)	RED% 28 DAYS	TOTAL MEAN AROMATICS (ppm)	RED% 28 DAYS
0	CONTROL NUTRIENT PRO-ACT	41277 41670 43193	0 0 0	6100 6140 6460	0 0 0
7	CONTROL NUTRIENT PRO-ACT	41393 26370 302	0 0 0	5705 3640 1979	0 0 0
28	CONTROL NUTRIENT PRO-ACT	42451 3654 59	-2.766 91.231 99.863	4546 1968 378	25.475 67.948 94.149

Results of Gravimetric Analysis:
Percentage (%) Decrease in Weight of Oil on Day 28

Control:
12.624%

Nutrient:
55.670%

Product:
65.516%

b. Toxicity:
NA

VIII. MICROBIOLOGICAL ANALYSIS
1. Listing of all microorganisms by species and percentages in the composition: CONFIDENTIAL 2. Optimum pH, temperature, and salinity ranges for use of the additive: pH: 7.0 Temperature: 30°C Salinity: 0.2 to 4% 3. Minimum and maximum pH, temperature, and salinity levels below or above which the effectiveness of the additive is reduced to half its optimum capacity: pH: <6.0 or >9.0 Temperature: <15°C or >45°C Salinity: <0.02% or >5% 4. Special nutrient requirements: PRO-ACT (aka, OILCLEAN w/ACTIVATOR) 5. Test results regarding the determination of the presence of the following: Salmonella: Negative Fecal coliform: Negative Shigella: Negative Staphylococcus Coagulase positive: Negative Beta hemolytic Streptococci: Negative

VIII. MICROBIOLOGICAL ANALYSIS

1. Listing of all microorganisms by species and percentages in the composition:
CONFIDENTIAL
2. Optimum pH, temperature, and salinity ranges for use of the additive:
pH: 7.0
Temperature: 30°C
Salinity: 0.2 to 4%
3. Minimum and maximum pH, temperature, and salinity levels below or above which the effectiveness of the additive is reduced to half its optimum capacity:
pH: <6.0 or >9.0
Temperature: <15°C or >45°C
Salinity: <0.02% or >5%
4. Special nutrient requirements: PRO-ACT (aka, OILCLEAN w/ACTIVATOR)
5. Test results regarding the determination of the presence of the following:
Salmonella: Negative
Fecal coliform: Negative
Shigella: Negative
Staphylococcus Coagulase positive: Negative
Beta hemolytic Streptococci: Negative

IX. PHYSICAL PROPERTIES
NA

X. ANALYSIS FOR HEAVY METALS, CYANIDE, AND CHLORINATED HYDROCARBONS
NA

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