SOIL RX

TECHNICAL PRODUCT BULLETIN: B-61
USEPA, OEM REGULATIONS IMPLEMENTATION DIVISION
LISTING DATE: NOVEMBER 17, 2010
“SOIL RX”

I. NAME, BRAND, OR TRADEMARK
SOIL RX (aka, BIO-REGEN HYDROCARBON) Type of Product: Bioremediation Agent (Microbiological Culture, Nutrient Additive)

II. NAME, ADDRESS, AND TELEPHONE NUMBER OF MANUFACTURER/CONTACT
3 Tier Technologies LLC Worldwide Headquarters 250 National Place, Suite 142 Longwood, FL 32750 Phone: (877) 226-7498 Fax: (877) 570-0072 E-mail: dburdette@3tiertech.com Web Site: http://www.3tiertech.com Exit (Daniel J. Burdette, President)

III. NAME, ADDRESS, AND TELEPHONE NUMBER OF PRIMARY DISTRIBUTORS
Direct Sales 3 Tier Technologies LLC Worldwide Headquarters 250 National Place, Suite 142 Longwood, FL 32750 Phone: (877) 226-7498 Fax: (877) 570-0072 E-mail: dburdette@3tiertech.com Web Site: http://www.3tiertech.com Exit (Daniel J. Burdette, President)

IV. SPECIAL HANDLING AND WORKER PRECAUTIONS FOR STORAGE AND FIELD APPLICATION
1. Flammability: Non-flammable 2. Ventilation: Normal ventilation is adequate. 3. Skin and eye contact; protective clothing; treatment in case of contact: Ingestion: No effect if ingested in small amounts. A single dose of this product is rarely toxic by ingestion. Irritation of the mouth, pharynx, esophagus and stomach can develop. Give two glasses of water. Do not induce vomiting. Contact physician if needed. Eyes: Avoid eye contact. This material may cause eye irritation. Immediately flush with water for 15 minutes. Contact physician if needed. Skin: Slight redness on hands and forearms if individual has a history of dermal allergic reactions. Dermatitis and skin sensitization can develop after repeated and/or prolonged contact. Remove contaminated clothing and footwear. Wash with soap and water. Always consult a physician if needed. Respirators are not required for recommended uses. Rubber gloves are not required, but recommended. Safety glasses are not required, but recommended. Wash hands with soap and water after handling product. Wash contaminated clothing and footwear before reuse. 4.a. Maximum storage temperature: 120°F 4.b. Minimum storage temperature: 34°F – do not allow product to freeze 4.c. Optimum storage temperature range: 45°F to 95°F. 4.d. Temperatures of phase separations and chemical changes: NA, stable.

IV. SPECIAL HANDLING AND WORKER PRECAUTIONS FOR STORAGE AND FIELD APPLICATION

1. Flammability: Non-flammable
2. Ventilation: Normal ventilation is adequate.
3. Skin and eye contact; protective clothing; treatment in case of contact: Ingestion: No effect if ingested in small amounts. A single dose of this product is rarely toxic by ingestion. Irritation of the mouth, pharynx, esophagus and stomach can develop. Give two glasses of water. Do not induce vomiting. Contact physician if needed. Eyes: Avoid eye contact. This material may cause eye irritation. Immediately flush with water for 15 minutes. Contact physician if needed. Skin: Slight redness on hands and forearms if individual has a history of dermal allergic reactions. Dermatitis and skin sensitization can develop after repeated and/or prolonged contact. Remove contaminated clothing and footwear. Wash with soap and water.

Always consult a physician if needed. Respirators are not required for recommended uses. Rubber gloves are not required, but recommended. Safety glasses are not required, but recommended. Wash hands with soap and water after handling product. Wash contaminated clothing and footwear before reuse.
4.a. Maximum storage temperature: 120°F
4.b. Minimum storage temperature: 34°F – do not allow product to freeze
4.c. Optimum storage temperature range: 45°F to 95°F.
4.d. Temperatures of phase separations and chemical changes: NA, stable.

V. SHELF LIFE
Shelf life exceeds 2 years when properly stored in the unopened original container.

VI. RECOMMENDED APPLICATION PROCEDURE
SOIL RX (aka, BIO-REGEN HYDROCARBON) is a concentrated liquid formulation of activated humic acid, highly concentrated hydrocarbon-oxidizing bacteria, and a readily biodegradable natural amino acid complex consisting of a nutrient-rich extract with a broad-spectrum package of identifiable amino acids, surfactants, and other proteins. This product is designed to digest organics and hydrocarbons in soil and water. 1. Application Method: SOIL RX (aka, BIO-REGEN HYDROCARBON) is a liquid concentrate that must be diluted prior to use. The product can be sprayed after dilution using standard spray application equipment, including but not limited to hand sprayers, mechanical sprayers, water trucks, fire or emergency response equipment, pressure washers, etc. Mix or saturate concentrate/water mixture with contaminated soils thoroughly for maximum performance. For shallow/surface contamination, drench affected areas with enough dilution to fully saturate the soil using normal spray equipment and water trucks. For general contamination less than two feet, contaminated soil may require tilling or excavation to properly mix concentrate/water dilution into soils. For deeper contamination greater than two feet, product application can be applied through boring the area and using perforated piping per regulatory recommendations or excavate the material and treat while land farming or bio-piling. For contaminated water, such as marshes, shoreline and open water with floating hydrocarbons, apply dilution directly to the contaminated areas using appropriate spray equipment and water cannons. 2. Concentration/Application Rate: SOIL RX (aka, BIO-REGEN HYDROCARBON) must be diluted using 1 part concentrate to 10 parts clean water prior to use. Product can be diluted up to 100 parts water as directed for specific applications. Application rates are determined by level of contamination, area of application, and speed required for cleanup. Specific application rates are determined prior to sale by the manufacturer and the distributor. A normal application rate for contaminated soil is on gallon 10:1 diluted product per cubic yard of soil. Normal application rate for water applications is three gallons 10:1 diluted product per 1000 square feet of contaminated surface area. 3. Conditions for Use: SOIL RX (aka, BIO-REGEN HYDROCARBON) is safe for application in ocean and fresh water, all soil types, and shoreline treatments. Water Salinity: Product should be diluted with fresh, clean water. Use of brackish or brine water may be used if fresh water is not available. It should be noted that the use of brine water may slightly reduce product performance or increase overall remediation time. Water Temperature: Water must be between 42° to 110°F for normal product performance. pH: Optimum water pH ranges between 4 to 8. Temperature: Temperatures are below 32° and above 120°F may decrease product performance. Nutrient Requirements: None. Nutrients required are in the product. Type and Age of Pollutants: For use on most organic based contaminants and hydrocarbon based materials like gasoline, jet fuels, diesel fuels, grease, tar, motor oils, crude oils, solvents, etc. Age of contamination is not a factor as much as level of contamination, depth of contamination, and length of time required for the remediation process. For materials that have hardened and are tar-like, additional procedures may be required for effective remediation.

VI. RECOMMENDED APPLICATION PROCEDURE

SOIL RX (aka, BIO-REGEN HYDROCARBON) is a concentrated liquid formulation of activated humic acid, highly concentrated hydrocarbon-oxidizing bacteria, and a readily biodegradable natural amino acid complex consisting of a nutrient-rich extract with a broad-spectrum package of identifiable amino acids, surfactants, and other proteins. This product is designed to digest organics and hydrocarbons in soil and water.
1. Application Method:
SOIL RX (aka, BIO-REGEN HYDROCARBON) is a liquid concentrate that must be diluted prior to use. The product can be sprayed after dilution using standard spray application equipment, including but not limited to hand sprayers, mechanical sprayers, water trucks, fire or emergency response equipment, pressure washers, etc. Mix or saturate concentrate/water mixture with contaminated soils thoroughly for maximum performance. For shallow/surface contamination, drench affected areas with enough dilution to fully saturate the soil using normal spray equipment and water trucks. For general contamination less than two feet, contaminated soil may require tilling or excavation to properly mix concentrate/water dilution into soils. For deeper contamination greater than two feet, product application can be applied through boring the area and using perforated piping per regulatory recommendations or excavate the material and treat while land farming or bio-piling. For contaminated water, such as marshes, shoreline and open water with floating hydrocarbons, apply dilution directly to the contaminated areas using appropriate spray equipment and water cannons.
2. Concentration/Application Rate:
SOIL RX (aka, BIO-REGEN HYDROCARBON) must be diluted using 1 part concentrate to 10 parts clean water prior to use. Product can be diluted up to 100 parts water as directed for specific applications. Application rates are determined by level of contamination, area of application, and speed required for cleanup. Specific application rates are determined prior to sale by the manufacturer and the distributor. A normal application rate for contaminated soil is on gallon 10:1 diluted product per cubic yard of soil. Normal application rate for water applications is three gallons 10:1 diluted product per 1000 square feet of contaminated surface area.
3. Conditions for Use:
SOIL RX (aka, BIO-REGEN HYDROCARBON) is safe for application in ocean and fresh water, all soil types, and shoreline treatments.

Water Salinity: Product should be diluted with fresh, clean water. Use of brackish or brine water may be used if fresh water is not available. It should be noted that the use of brine water may slightly reduce product performance or increase overall remediation time.
Water Temperature: Water must be between 42° to 110°F for normal product performance.
pH: Optimum water pH ranges between 4 to 8.
Temperature: Temperatures are below 32° and above 120°F may decrease product performance.
Nutrient Requirements: None. Nutrients required are in the product.
Type and Age of Pollutants: For use on most organic based contaminants and hydrocarbon based materials like gasoline, jet fuels, diesel fuels, grease, tar, motor oils, crude oils, solvents, etc. Age of contamination is not a factor as much as level of contamination, depth of contamination, and length of time required for the remediation process. For materials that have hardened and are tar-like, additional procedures may be required for effective remediation.

VII. TOXICITY AND EFFECTIVENESS

a. Effectiveness:
Bioremediation Agent Effectiveness Test (40 CFR 300.900), Federal Register September 15, 1994:
Summary Data Table

DAYS	PRODUCT 3 REPS/PROD	TOTAL MEAN ALKANES (ppm)	RED% 28 DAYS	TOTAL MEAN AROMATICS (ppm)	RED% 28 DAYS
0	CONTROL NUTRIENT SOIL RX	34908.9 34863.3 34993.0	0 0 0	6051.3 6047.4 6069.0	0 0 0
7	CONTROL NUTRIENT SOIL RX	35206.2 32894.1 24446.0	+0.08 5.64 30.14	5720.9 5745.8 3219.2	5.45 4.98 46.96
28	CONTROL NUTRIENT SOIL RX	34287.8 32610.0 17597.0	1.779 6.463 49.713	5892.7 5205.1 1600.1	2.621 13.928 73.635

Results of Gravimetric Analysis:
Percentage (%) Decrease in Weight of Oil on Day 28

Control:
8.01%

Nutrient:
7.43%

Product:
30.14%

b. Toxicity:
NA

VIII. MICROBIOLOGICAL ANALYSIS
1. Listing of all microorganisms by species and percentages in the composition: CONFIDENTIAL 2. Optimum pH, temperature, and salinity ranges for use of the additive: pH: 4-8 Temperature: 42°F-100°F Salinity: NA 3. Minimum and maximum pH, temperature, and salinity levels below or above which the effectiveness of the additive is reduced to half its optimum capacity: pH: 2-10 Temperature: 34°F-125°F Salinity: NA 4. Special nutrient requirements: None 5. Test results regarding the determination of the presence of the following: Salmonella: Negative Fecal coliform: Negative Shigella: Negative Staphylococcus Coagulase positive: Negative Beta hemolytic Streptococci: Negative

VIII. MICROBIOLOGICAL ANALYSIS

1. Listing of all microorganisms by species and percentages in the composition:
CONFIDENTIAL
2. Optimum pH, temperature, and salinity ranges for use of the additive:
pH: 4-8
Temperature: 42°F-100°F
Salinity: NA
3. Minimum and maximum pH, temperature, and salinity levels below or above which the effectiveness of the additive is reduced to half its optimum capacity:
pH: 2-10
Temperature: 34°F-125°F
Salinity: NA
4. Special nutrient requirements: None
5. Test results regarding the determination of the presence of the following:
Salmonella: Negative
Fecal coliform: Negative
Shigella: Negative
Staphylococcus Coagulase positive: Negative
Beta hemolytic Streptococci: Negative

IX. PHYSICAL PROPERTIES
NA

X. ANALYSIS FOR HEAVY METALS, CYANIDE, AND CHLORINATED HYDROCARBONS
NA

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