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See: Sample Collection Information Document for Pathogens - Companion to Selected Analytical Methods for Environmental Remediation and Recovery (SAM) 2017.
General Notes:
For SAM 2017 methods, see: Selected Analytical Methods for Environmental Remediation and Recovery (SAM).
Analytical technologies and methods addressing biotoxins continue to be developed and improved; the contact information on this website should be used for updates regarding analytical procedures.
Sample sizes are provided for guidance, and may vary depending on the specific contamination incident, data quality objectives and requirements, and laboratory needs. If requested by the laboratory, additional sample(s) must be collected for analyses using multiple methods, or for laboratory quality control analyses (e.g., duplicates, matrix spikes). It is also recommended that additional sample(s) be collected in cases where low concentrations are expected or in the case of an anticipated need for reanalysis due to sample spillage or unforseen analytical difficulties.
| Analyte: |
Abrin and Abrine (abrin marker)
|
|---|
| Sample Type: |
Aerosol (filter/cassette, liquid impinger)
|
|---|
| Sampling Device/Medium: |
Filter: Sterile MCE or PTFE filter. Place the filter in a sterile leak proof container.
Impinger: Transfer contents into sterile leak proof container for shipping.
|
|---|
| Sample Container Size: |
Volumes of collected air samples are dependent on the concentration of the toxin and the collection device used. Sample collectors should consult the incident-specific Sample Collection Plan (SCP), Health and Safety Plan (HASP) and/or analytical laboratory for appropriate volumes.
|
|---|
| Preservation: |
• Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
• Avoid freeze-thaw cycles during transport of samples collected for abrin.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
NOTE: 28 days for abrine in water
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, liquid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
Information regarding the types of filters used for collection of aerosol samples to be analyzed for toxins is limited. Sample collectors also should consult the Sample Collection Plan (SCP), Health and Safety Plan (HASP) and/or analytical laboratory to determine the appropriate air collection filter(s).
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [LFA] Adapted from Biosecurity and Bioterrorism: Biodefense Strategy, Practice, and Science (2014) 12(1): 49-62 [Tier I / Tier II]
• as Abrine [LC-MS-MS] EPA 600/R-13/022 [Tier I / Tier II]
Confirmatory:
• [ELISA and ECL] Adapted from Journal of Food Protection (2008) 71(9): 1868-1874 [Tier II]
Biological Activity:
• [Enzyme Activity] Adapted from Analytical Biochemistry (2008) 378(1): 87-89 [Tier II]
|
|---|
| Analyte: |
Abrin and Abrine (abrin marker)
|
|---|
| Sample Type: |
Particulate (swabs, wipes, dust socks)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container
|
|---|
| Sample Container Size: |
At least 2 sterile, synthetic, and moistened wipes, swabs, or dust socks
|
|---|
| Preservation: |
• Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
• Avoid freeze-thaw cycles during transport of samples collected for abrin.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, liquid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [LFA] Adapted from Biosecurity and Bioterrorism: Biodefense Strategy, Practice, and Science (2014) 12(1): 49-62 [Tier I / Tier II]
• as Abrine [LC-MS-MS] EPA 600/R-13/022 [Tier I / Tier II]
Confirmatory:
• [ELISA and ECL] Adapted from Journal of Food Protection (2008) 71(9): 1868-1874 [Tier II]
Biological Activity:
• [Enzyme Activity] Adapted from Analytical Biochemistry (2008) 378(1): 87-89 [Tier II]
|
|---|
| Analyte: |
Abrin and Abrine (abrin marker)
|
|---|
| Sample Type: |
Solid (soil, powder)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container
|
|---|
| Sample Container Size: |
50 - 100 mL
|
|---|
| Preservation: |
• Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
• Avoid freeze-thaw cycles during transport of samples collected for abrin.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, liquid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [LFA] Adapted from Biosecurity and Bioterrorism: Biodefense Strategy, Practice, and Science (2014) 12(1): 49-62 [Tier I / Tier II]
• as Abrine [LC-MS-MS] EPA 600/R-13/022 [Tier I / Tier II]
Confirmatory:
• [ELISA and ECL] Adapted from Journal of Food Protection (2008) 71(9): 1868-1874 [Tier II]
Biological Activity:
• [Enzyme Activity] Adapted from Analytical Biochemistry (2008) 378(1): 87-89 [Tier II]
|
|---|
| Analyte: |
Abrin and Abrine (abrin marker)
|
|---|
| Sample Type: |
Water (surface water, waste water, drinking water)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container
NOTE: Collect water samples for analysis of abrine in polypropylene container with polyethylene screw-cap.
|
|---|
| Sample Container Size: |
100 mL
|
|---|
| Preservation: |
• Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
• Avoid freeze-thaw cycles during transport of samples collected for abrin.
NOTE: For detection and measurement of abrine in treated water samples, add the following to the sample container prior to collection:
• 8 mg sodium thiosulfate/100-mL sample
• 6.4 mg sodium omadine /100-mL sample
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
NOTE: 28 days for abrine in water
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, liquid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [LFA] Adapted from Biosecurity and Bioterrorism: Biodefense Strategy, Practice, and Science (2014) 12(1): 49-62 [Tier I / Tier II]
• as Abrine [LC-MS-MS] EPA 600/R-13/022 [Tier I / Tier II]
Confirmatory:
• [ELISA and ECL] Adapted from Journal of Food Protection (2008) 71(9): 1868-1874 [Tier II]
Biological Activity:
• [Enzyme Activity] Adapted from Analytical Biochemistry (2008) 378(1): 87-89 [Tier II]
|
|---|
| Analyte: |
Aflatoxins (B1, B2, G1, G2)
|
|---|
| Sample Type: |
Solid (soil, powder)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container
|
|---|
| Sample Container Size: |
50 - 100 mL
|
|---|
| Preservation: |
Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group I
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, solid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive and Confirmatory:
[Immunoaffinity (column) purification / HPLC-FL] Adapted from 991.31 (AOAC) [Tier II]
|
|---|
| Analyte: |
Aflatoxins (B1, B2, G1, G2)
|
|---|
| Sample Type: |
Particulate (swabs, wipes, dust socks)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container
|
|---|
| Sample Container Size: |
At least 2 sterile, synthetic, and moistened wipes, swabs, or dust socks
|
|---|
| Preservation: |
Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group I
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, solid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive and Confirmatory:
[Immunoaffinity (column) purification / HPLC-FL] Adapted from 991.31 (AOAC) [Tier II]
|
|---|
| Analyte: |
Aflatoxins (B1, B2, G1, G2)
|
|---|
| Sample Type: |
Water (surface water, waste water, drinking water)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container
|
|---|
| Sample Container Size: |
100 mL
|
|---|
| Preservation: |
Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group I
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, solid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive and Confirmatory:
[Immunoaffinity (column) purification / HPLC-FL] Adapted from 991.31 (AOAC) [Tier II]
|
|---|
| Analyte: |
Aflatoxins (B1, B2, G1, G2)
|
|---|
| Sample Type: |
Aerosol (filter/cassette, liquid impinger)
|
|---|
| Sampling Device/Medium: |
Filter: Sterile MCE or PTFE filter. Place the filter in a sterile leak proof container.
Impinger: Transfer contents into sterile leak proof container for shipping.
|
|---|
| Sample Container Size: |
Volumes of collected air samples are dependent on the concentration of the toxin and the collection device used. Sample collectors should consult the incident-specific Sample Collection Plan (SCP), Health and Safety Plan (HASP) and/or analytical laboratory for appropriate volumes.
|
|---|
| Preservation: |
Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group I
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, solid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
Information regarding the types of filters used for collection of aerosol samples to be analyzed for toxins is limited. Sample collectors also should consult the Sample Collection Plan (SCP), Health and Safety Plan (HASP) and/or analytical laboratory to determine the appropriate air collection filter(s).
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive and Confirmatory:
[Immunoaffinity (column) purification / HPLC-FL] Adapted from 991.31 (AOAC) [Tier II]
|
|---|
| Analyte: |
Anatoxin-a
|
|---|
| Sample Type: |
Particulate (swabs, wipes, dust socks)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container - amber glass or amber PETG (or protect from light)
|
|---|
| Sample Container Size: |
At least 2 sterile, synthetic, and moistened wipes, swabs, or dust socks
|
|---|
| Preservation: |
• Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
• Protect from light
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group II
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, liquid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• ELISA [Tier III] (No reference is available at this time. Please consult the SAM website* for information regarding the status of this assay.)
Confirmatory:
• [LC-ESI-MS-MS] Method 545 (EPA) [Tier I / Tier II]
|
|---|
| Analyte: |
Anatoxin-a
|
|---|
| Sample Type: |
Solid (soil, powder)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container - amber glass or amber PETG (or protect from light)
|
|---|
| Sample Container Size: |
50 - 100 mL
|
|---|
| Preservation: |
• Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
• Protect from light
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group II
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, liquid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• ELISA [Tier III] (No reference is available at this time. Please consult the SAM website* for information regarding the status of this assay.)
Confirmatory:
• [LC-ESI-MS-MS] Method 545 (EPA) [Tier I / Tier II]
|
|---|
| Analyte: |
Anatoxin-a
|
|---|
| Sample Type: |
Aerosol (filter/cassette, liquid impinger)
|
|---|
| Sampling Device/Medium: |
Filter: Sterile MCE or PTFE filter. Place the filter in a sterile leak proof container.
Impinger: Transfer contents into sterile leak proof container for shipping.
NOTE: Use amber glass or amber PETG containers (or protect from light)
|
|---|
| Sample Container Size: |
Volumes of collected air samples are dependent on the concentration of the toxin and the collection device used. Sample collectors should consult the incident-specific Sample Collection Plan (SCP), Health and Safety Plan (HASP) and/or analytical laboratory for appropriate volumes.
|
|---|
| Preservation: |
• Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
• Protect from light
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group II
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, liquid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
Information regarding the types of filters used for collection of aerosol samples to be analyzed for toxins is limited. Sample collectors also should consult the Sample Collection Plan (SCP), Health and Safety Plan (HASP) and/or analytical laboratory to determine the appropriate air collection filter(s).
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• ELISA [Tier III] (No reference is available at this time. Please consult the SAM website* for information regarding the status of this assay.)
Confirmatory:
• [LC-ESI-MS-MS] Method 545 (EPA) [Tier I / Tier II]
|
|---|
| Analyte: |
Anatoxin-a
|
|---|
| Sample Type: |
Water (surface water, waste water, drinking water)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container - amber glass or amber PETG (or protect from light)
|
|---|
| Sample Container Size: |
100 mL
|
|---|
| Preservation: |
• Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
• Protect from light
NOTE: For treated water samples, add 1 g sodium bisulfate and 0.1 g ascorbic acid / 1-L sample.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
NOTE: 28 days for water samples cooled to < 10°C
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group II
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, liquid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• ELISA [Tier III] (No reference is available at this time. Please consult the SAM website* for information regarding the status of this assay.)
Confirmatory:
• [LC-ESI-MS-MS] Method 545 (EPA) [Tier I / Tier II]
|
|---|
| Analyte: |
Botulinum neurotoxins (Serotypes A - G)
|
|---|
| Sample Type: |
Particulate (swabs, wipes, dust socks)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container
|
|---|
| Sample Container Size: |
At least 2 sterile, synthetic, and moistened wipes, swabs, or dust socks
|
|---|
| Preservation: |
• Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
• Avoid freeze-thaw cycles during transport.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group I
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, liquid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [LFA] Adapted from EPA Environmental Technology Verification report [Tier I / Tier II]
• [ECL] Adapted from Journal of the Science of Food and Agriculture (2014) 94: 707-712 [Tier II]
• [Fluorescent bead-based assay] Multi-agency Report. Rapid Botulinum Toxin Assay Test, Evaluation and Validation Study Report [Tier III]
• [Antibody capture FRET-based activity] Adapted from Analytical Biochemistry (2011) 411: 200-209 [Tier II]
Confirmatory:
• [LC-ESI-MS-MS / MALDI-TOF MS] Adapted from Analytical Chemistry (2005) 77: 3916-3924 [Tier II]
Biological Activity:
• [Mouse Bioassay] APHA Press Compendium of Methods, Chapter 32 [Tier I]
|
|---|
| Analyte: |
Botulinum neurotoxins (Serotypes A - G)
|
|---|
| Sample Type: |
Aerosol (filter/cassette, liquid impinger)
|
|---|
| Sampling Device/Medium: |
Filter: Sterile MCE or PTFE filter. Place the filter in a sterile leak proof container.
Impinger: Transfer contents into sterile leak proof container for shipping.
|
|---|
| Sample Container Size: |
Volumes of collected air samples are dependent on the concentration of the toxin and the collection device used. Sample collectors should consult the incident-specific Sample Collection Plan (SCP), Health and Safety Plan (HASP) and/or analytical laboratory for appropriate volumes.
|
|---|
| Preservation: |
• Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
• Avoid freeze-thaw cycles during transport.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group I
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, liquid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
Information regarding the types of filters used for collection of aerosol samples to be analyzed for toxins is limited. Sample collectors also should consult the Sample Collection Plan (SCP), Health and Safety Plan (HASP) and/or analytical laboratory to determine the appropriate air collection filter(s).
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [LFA] Adapted from EPA Environmental Technology Verification report [Tier I / Tier II]
• [ECL] Adapted from Journal of the Science of Food and Agriculture (2014) 94: 707-712 [Tier II]
• [Fluorescent bead-based assay] Multi-agency Report. Rapid Botulinum Toxin Assay Test, Evaluation and Validation Study Report [Tier III]
• [Antibody capture FRET-based activity] Adapted from Analytical Biochemistry (2011) 411: 200-209 [Tier II]
Confirmatory:
• [LC-ESI-MS-MS / MALDI-TOF MS] Adapted from Analytical Chemistry (2005) 77: 3916-3924 [Tier II]
Biological Activity:
• [Mouse Bioassay] APHA Press Compendium of Methods, Chapter 32 [Tier I]
|
|---|
| Analyte: |
Botulinum neurotoxins (Serotypes A - G)
|
|---|
| Sample Type: |
Solid (soil, powder)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container
|
|---|
| Sample Container Size: |
50 - 100 mL
|
|---|
| Preservation: |
• Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
• Avoid freeze-thaw cycles during transport.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group I
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, liquid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [LFA] Adapted from EPA Environmental Technology Verification report [Tier I / Tier II]
• [ECL] Adapted from Journal of the Science of Food and Agriculture (2014) 94: 707-712 [Tier II]
• [Fluorescent bead-based assay] Multi-agency Report. Rapid Botulinum Toxin Assay Test, Evaluation and Validation Study Rep
• [Antibody capture FRET-based activity] Adapted from Analytical Biochemistry (2011) 411: 200-209 [Tier II]
Confirmatory:
• [LC-ESI-MS-MS / MALDI-TOF MS] Adapted from Analytical Chemistry (2005) 77: 3916-3924 [Tier II]
Biological Activity:
• [Mouse Bioassay] APHA Press Compendium of Methods, Chapter 32 [Tier I]
|
|---|
| Analyte: |
Botulinum neurotoxins (Serotypes A - G)
|
|---|
| Sample Type: |
Water (surface water, waste water, drinking water)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container
|
|---|
| Sample Container Size: |
100 mL
|
|---|
| Preservation: |
• Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
• Avoid freeze-thaw cycles during transport.
NOTE: For treated water, add 50 mg sodium thiosulfate / L sample
(DO NOT add sodium thiosulfate to samples that will be analyzed using MS)
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group I
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, liquid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [LFA] Adapted from EPA Environmental Technology Verification report [Tier I / Tier II]
• [ECL] Adapted from Journal of the Science of Food and Agriculture (2014) 94: 707-712 [Tier II]
• [Fluorescent bead-based assay] Multi-agency Report. Rapid Botulinum Toxin Assay Test, Evaluation and Validation Study Report [Tier III]
• [Antibody capture FRET-based activity] Adapted from Analytical Biochemistry (2011) 411: 200-209 [Tier II]
Confirmatory:
• [LC-ESI-MS-MS / MALDI-TOF MS] Adapted from Analytical Chemistry (2005) 77: 3916-3924 [Tier II]
Biological Activity:
• [Mouse Bioassay] APHA Press Compendium of Methods, Chapter 32 [Tier I]
|
|---|
| Analyte: |
Brevetoxins (A and B Forms)
|
|---|
| Sample Type: |
Water (surface water, waste water, drinking water)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container
|
|---|
| Sample Container Size: |
100 mL
|
|---|
| Preservation: |
Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group II
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxic solids, organic, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive and Confirmatory:
• [ELISA / LC-MS] Adapted from Toxicon (2015) 96: 82-88 [Tier II]
|
|---|
| Analyte: |
Brevetoxins (A and B Forms)
|
|---|
| Sample Type: |
Solid (soil, powder)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container
|
|---|
| Sample Container Size: |
50 - 100 mL
|
|---|
| Preservation: |
Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group II
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxic solids, organic, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive and Confirmatory:
• [ELISA / LC-MS] Adapted from Toxicon (2015) 96: 82-88 [Tier II]
|
|---|
| Analyte: |
Brevetoxins (A and B Forms)
|
|---|
| Sample Type: |
Particulate (swabs, wipes, dust socks)
|
|---|
| Sampling Device/Medium: |
Sterile, leak proof container
|
|---|
| Sample Container Size: |
At least 2 sterile, synthetic, and moistened wipes, swabs, or dust socks
|
|---|
| Preservation: |
Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group II
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxic solids, organic, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive and Confirmatory:
• [ELISA / LC-MS] Adapted from Toxicon (2015) 96: 82-88 [Tier II]
|
|---|
| Analyte: |
Brevetoxins (A and B Forms)
|
|---|
| Sample Type: |
Aerosol (filter/cassette, liquid impinger)
|
|---|
| Sampling Device/Medium: |
Filter: Sterile MCE or PTFE filter. Place the filter in a sterile leak proof container.
Impinger: Transfer contents into sterile leak proof container for shipping.
|
|---|
| Sample Container Size: |
Volumes of collected air samples are dependent on the concentration of the toxin and the collection device used. Sample collectors should consult the incident-specific Sample Collection Plan (SCP), Health and Safety Plan (HASP) and/or analytical laboratory for appropriate volumes.
|
|---|
| Preservation: |
Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group II
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxic solids, organic, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
Information regarding the types of filters used for collection of aerosol samples to be analyzed for toxins is limited. Sample collectors also should consult the Sample Collection Plan (SCP), Health and Safety Plan (HASP) and/or analytical laboratory to determine the appropriate air collection filter(s).
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive and Confirmatory:
• [ELISA / LC-MS] Adapted from Toxicon (2015) 96: 82-88 [Tier II]
|
|---|
| Analyte: |
Cylindrospermopsin
|
|---|
| Sample Type: |
Water (surface water, waste water, drinking water)
|
|---|
| Sampling Device/Medium: |
Amber glass container with fluoropolymer- lined screw cap
|
|---|
| Sample Container Size: |
100 mL
|
|---|
| Preservation: |
Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
NOTE: For treated water, add 1 g sodium bisulfate and 0.1 g ascorbic acid / 1-L sample.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
NOTE: 28 days for water samples cooled to < 10°C
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
|
|---|
| Shipping Label: |
This substance does not require specific labeling for transport.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [ELISA] Adapted from Environmental Sciences and Technology (2010) 44: 7361-7368 [Tier II]
Confirmatory:
• [LC-ESI-MS-MS] Method 545 (EPA) [Tier I / Tier II]
|
|---|
| Analyte: |
Cylindrospermopsin
|
|---|
| Sample Type: |
Particulate (swabs, wipes, dust socks)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container - amber glass or amber PETG (or protect from light)
|
|---|
| Sample Container Size: |
At least 2 sterile, synthetic, and moistened wipes, swabs, or dust socks
|
|---|
| Preservation: |
Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
|
|---|
| Shipping Label: |
This substance does not require specific labeling for transport.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [ELISA] Adapted from Environmental Sciences and Technology (2010) 44: 7361-7368 [Tier II]
Confirmatory:
• [LC-ESI-MS-MS] Method 545 (EPA) [Tier I / Tier II]
|
|---|
| Analyte: |
Cylindrospermopsin
|
|---|
| Sample Type: |
Solid (soil, powder)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container - amber glass or amber PETG (or protect from light)
|
|---|
| Sample Container Size: |
50 - 100 mL
|
|---|
| Preservation: |
Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
|
|---|
| Shipping Label: |
This substance does not require specific labeling for transport.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [ELISA] Adapted from Environmental Sciences and Technology (2010) 44: 7361-7368 [Tier II]
Confirmatory:
• [LC-ESI-MS-MS] Method 545 (EPA) [Tier I / Tier II]
|
|---|
| Analyte: |
Cylindrospermopsin
|
|---|
| Sample Type: |
Aerosol (filter/cassette, liquid impinger)
|
|---|
| Sampling Device/Medium: |
Filter: Sterile MCE or PTFE filter. Place the filter in a sterile leak proof container.
Impinger: Transfer contents into sterile leak proof container for shipping.
NOTE: Use amber glass or amber PETG containers (or protect from light)
|
|---|
| Sample Container Size: |
Volumes of collected air samples are dependent on the concentration of the toxin and the collection device used. Sample collectors should consult the incident-specific Sample Collection Plan (SCP), Health and Safety Plan (HASP) and/or analytical laboratory for appropriate volumes.
|
|---|
| Preservation: |
Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
|
|---|
| Shipping Label: |
This substance does not require specific labeling for transport.
|
|---|
| Notes: |
Information regarding the types of filters used for collection of aerosol samples to be analyzed for toxins is limited. Sample collectors also should consult the Sample Collection Plan (SCP), Health and Safety Plan (HASP) and/or analytical laboratory to determine the appropriate air collection filter(s).
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [ELISA] Adapted from Environmental Sciences and Technology (2010) 44: 7361-7368 [Tier II]
Confirmatory:
• [LC-ESI-MS-MS] Method 545 (EPA) [Tier I / Tier II]
|
|---|
| Analyte: |
Diacetoxyscirpenol (DAS)
|
|---|
| Sample Type: |
Aerosol (filter/cassette, liquid impinger)
|
|---|
| Sampling Device/Medium: |
Filter: Sterile MCE or PTFE filter. Place the filter in a sterile leak proof container.
Impinger: Transfer contents into sterile leak proof container for shipping.
|
|---|
| Sample Container Size: |
Volumes of collected air samples are dependent on the concentration of the toxin and the collection device used. Sample collectors should consult the incident-specific Sample Collection Plan (SCP), Health and Safety Plan (HASP) and/or analytical laboratory for appropriate volumes.
|
|---|
| Preservation: |
Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group II
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, solid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
Information regarding the types of filters used for collection of aerosol samples to be analyzed for toxins is limited. Sample collectors also should consult the Sample Collection Plan (SCP), Health and Safety Plan (HASP) and/or analytical laboratory to determine the appropriate air collection filter(s).
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [ELISA] Adapted from Journal of Food Microbiology (1988) 6(1): 9-17 [Tier III]
Confirmatory:
• [LC-MS-MS] Adapted from Food Research International (2015) 72: 247-255 [Tier II]
|
|---|
| Analyte: |
Diacetoxyscirpenol (DAS)
|
|---|
| Sample Type: |
Particulate (swabs, wipes, dust socks)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container
|
|---|
| Sample Container Size: |
At least 2 sterile, synthetic, and moistened wipes, swabs, or dust socks
|
|---|
| Preservation: |
Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group II
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, solid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [ELISA] Adapted from Journal of Food Microbiology (1988) 6(1): 9-17 [Tier III]
Confirmatory:
• [LC-MS-MS] Adapted from Food Research International (2015) 72: 247-255 [Tier II]
|
|---|
| Analyte: |
Diacetoxyscirpenol (DAS)
|
|---|
| Sample Type: |
Solid (soil, powder)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container
|
|---|
| Sample Container Size: |
50 - 100 mL
|
|---|
| Preservation: |
Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group II
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, solid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [ELISA] Adapted from Journal of Food Microbiology (1988) 6(1): 9-17 [Tier III]
Confirmatory:
• [LC-MS-MS] Adapted from Food Research International (2015) 72: 247-255 [Tier II]
|
|---|
| Analyte: |
Diacetoxyscirpenol (DAS)
|
|---|
| Sample Type: |
Water (surface water, waste water, drinking water)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container
|
|---|
| Sample Container Size: |
100 mL
|
|---|
| Preservation: |
Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group II
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials:
Label outer package with the proper shipping name: "Toxins, extracted from living sources, solid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [ELISA] Adapted from Journal of Food Microbiology (1988) 6(1): 9-17 [Tier III]
Confirmatory:
• [LC-MS-MS] Adapted from Food Research International (2015) 72: 247-255 [Tier II]
|
|---|
| Analyte: |
Domoic acid
|
|---|
| Sample Type: |
Aerosol (filter/cassette, liquid impinger)
|
|---|
| Sampling Device/Medium: |
Filter: Sterile MCE or PTFE filter. Place the filter in a sterile leak proof container.
Impinger: Transfer contents into sterile leak proof container for shipping.
|
|---|
| Sample Container Size: |
Volumes of collected air samples are dependent on the concentration of the toxin and the collection device used. Sample collectors should consult the incident-specific Sample Collection Plan (SCP), Health and Safety Plan (HASP) and/or analytical laboratory for appropriate volumes.
|
|---|
| Preservation: |
Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
|
|---|
| Shipping Label: |
This substance does not require specific labeling for transport.
|
|---|
| Notes: |
Information regarding the types of filters used for collection of aerosol samples to be analyzed for toxins is limited. Sample collectors also should consult the Sample Collection Plan (SCP), Health and Safety Plan (HASP) and/or analytical laboratory to determine the appropriate air collection filter(s).
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [ELISA] Adapted from Journal of AOAC International (2007) 90(4): 1011-1027 [Tier II]
• [ELISA] Adapted from Journal of Shellfish Research (2008) 27(5): 1301-1310 [Tier II]
Confirmatory:
• [LC-UV] Adapted from Journal of the Mexican Chemical Society (2011) 55(2): 65-71 [Tier II]
|
|---|
| Analyte: |
Domoic acid
|
|---|
| Sample Type: |
Solid (soil, powder)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container
|
|---|
| Sample Container Size: |
50 - 100 mL
|
|---|
| Preservation: |
Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
|
|---|
| Shipping Label: |
This substance does not require specific labeling for transport.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [ELISA] Adapted from Journal of AOAC International (2007) 90(4): 1011-1027 [Tier II]
• [ELISA] Adapted from Journal of Shellfish Research (2008) 27(5): 1301-1310 [Tier II]
Confirmatory:
• [LC-UV] Adapted from Journal of the Mexican Chemical Society (2011) 55(2): 65-71 [Tier II]
|
|---|
| Analyte: |
Domoic acid
|
|---|
| Sample Type: |
Water (surface water, waste water, drinking water)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container
|
|---|
| Sample Container Size: |
A minimum of 100 mL
|
|---|
| Preservation: |
Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
|
|---|
| Shipping Label: |
This substance does not require specific labeling for transport.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [ELISA] Adapted from Journal of AOAC International (2007) 90(4): 1011-1027 [Tier II]
• [ELISA] Adapted from Journal of Shellfish Research (2008) 27(5): 1301-1310 [Tier II]
Confirmatory:
• [LC-UV] Adapted from Journal of the Mexican Chemical Society (2011) 55(2): 65-71 [Tier II]
|
|---|
| Analyte: |
Domoic acid
|
|---|
| Sample Type: |
Particulate (swabs, wipes, dust socks)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container
|
|---|
| Sample Container Size: |
At least 2 sterile, synthetic, and moistened wipes, swabs, or dust socks
|
|---|
| Preservation: |
Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
|
|---|
| Shipping Label: |
This substance does not require specific labeling for transport.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [ELISA] Adapted from Journal of AOAC International (2007) 90(4): 1011-1027 [Tier II]
• [ELISA] Adapted from Journal of Shellfish Research (2008) 27(5): 1301-1310 [Tier II]
Confirmatory:
• [LC-UV] Adapted from Journal of the Mexican Chemical Society (2011) 55(2): 65-71 [Tier II]
|
|---|
| Analyte: |
Microcystins (LA, LF, LR, LY, RR, YR)
|
|---|
| Sample Type: |
Solid (soil, powder)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container - amber glass or amber PETG (or protect from light)
|
|---|
| Sample Container Size: |
50 - 100 mL
|
|---|
| Preservation: |
• Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C; do not freeze]
• Protect from light
|
|---|
| Holding Time: |
Method 544 analysis: 28 days
Method 546 analysis: 48 hours (14 days if frozen within 48 hours)
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group I
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, solid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [ELISA] Method 546 (EPA) [Tier I / Tier II]
Confirmatory:
• [LC-MS-MS] Method 544 (EPA) [Tier I / Tier II]
|
|---|
| Analyte: |
Microcystins (LA, LF, LR, LY, RR, YR)
|
|---|
| Sample Type: |
Particulate (swabs, wipes, dust socks)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container - amber glass or amber PETG (or protect from light)
|
|---|
| Sample Container Size: |
At least 2 sterile, synthetic, and moistened wipes, swabs, or dust socks
|
|---|
| Preservation: |
• Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C; do not freeze]
• Protect from light
|
|---|
| Holding Time: |
Method 544 analysis: 28 days
Method 546 analysis: 48 hours (14 days if frozen within 48 hours)
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group I
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, solid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [ELISA] Method 546 (EPA) [Tier I / Tier II]
Confirmatory:
• [LC-MS-MS] Method 544 (EPA) [Tier I / Tier II]
|
|---|
| Analyte: |
Microcystins (LA, LF, LR, LY, RR, YR)
|
|---|
| Sample Type: |
Water (surface water, waste water, drinking water)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container - amber glass or amber PETG (or protect from light)
|
|---|
| Sample Container Size: |
500 mL
|
|---|
| Preservation: |
• Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C; do not freeze]
• Protect from light
NOTE:
Prior to collection, add the following to containers used to collect treated water samples:
• For samples to be analyzed using ELISA (Method 546), 100 mg sodium thiosulfate / 1-L sample.
• For samples to be analyzed using LC-MS-MS (Method 544), add 1) 7.75 g Trizma, 2) 2 g 2-chloroacetamide, 3) 100 mg ascorbic acid, 4) 0.35 g ethylenediamine-tetraacetic acid trisodium salt / 1-L sample.
|
|---|
| Holding Time: |
Method 544 analysis: 28 days
Method 546 analysis: 48 hours (14 days if frozen within 48 hours)
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group I
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, solid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [ELISA] Method 546 (EPA) [Tier I / Tier II]
Confirmatory:
• [LC-MS-MS] Method 544 (EPA) [Tier I / Tier II]
|
|---|
| Analyte: |
Microcystins (LA, LF, LR, LY, RR, YR)
|
|---|
| Sample Type: |
Aerosol (filter/cassette, liquid impinger)
|
|---|
| Sampling Device/Medium: |
Filter: Sterile MCE or PTFE filter. Place the filter in a sterile leak proof container.
Impinger: Transfer contents into sterile leak proof container for shipping.
NOTE: Use amber glass or amber PETG containers (or protect from light)
|
|---|
| Sample Container Size: |
Volumes of collected air samples are dependent on the concentration of the toxin and the collection device used. Sample collectors should consult the incident-specific Sample Collection Plan (SCP), Health and Safety Plan (HASP) and/or analytical laboratory for appropriate volumes.
|
|---|
| Preservation: |
• Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C; do not freeze]
• Protect from light
|
|---|
| Holding Time: |
Method 544 analysis: 28 days
Method 546 analysis: 48 hours (14 days if frozen within 48 hours)
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group I
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, solid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
Information regarding the types of filters used for collection of aerosol samples to be analyzed for toxins is limited. Sample collectors also should consult the Sample Collection Plan (SCP), Health and Safety Plan (HASP) and/or analytical laboratory to determine the appropriate air collection filter(s).
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [ELISA] Method 546 (EPA) [Tier I / Tier II]
Confirmatory:
• [LC-MS-MS] Method 544 (EPA) [Tier I / Tier II]
|
|---|
| Analyte: |
Picrotoxin
|
|---|
| Sample Type: |
Water (surface water, waste water, drinking water)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container
|
|---|
| Sample Container Size: |
100 mL
|
|---|
| Preservation: |
• Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
• Protect from light.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group II
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, solid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Confirmatory:
• [LC-UV] Adapted from Journal of Pharmaceutical and Biomedical Analysis (1989) 7(3): 369-375 [Tier II]
|
|---|
| Analyte: |
Picrotoxin
|
|---|
| Sample Type: |
Solid (soil, powder)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container
|
|---|
| Sample Container Size: |
50 - 100 mL
|
|---|
| Preservation: |
• Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
• Protect from light.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group II
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, solid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Confirmatory:
• [LC-UV] Adapted from Journal of Pharmaceutical and Biomedical Analysis (1989) 7(3): 369-375 [Tier II]
|
|---|
| Analyte: |
Picrotoxin
|
|---|
| Sample Type: |
Aerosol (filter/cassette, liquid impinger)
|
|---|
| Sampling Device/Medium: |
Filter: Sterile MCE or PTFE filter. Place the filter in a sterile leak proof container.
Impinger: Transfer contents into sterile leak proof container for shipping.
|
|---|
| Sample Container Size: |
Volumes of collected air samples are dependent on the concentration of the toxin and the collection device used. Sample collectors should consult the incident-specific Sample Collection Plan (SCP), Health and Safety Plan (HASP) and/or analytical laboratory for appropriate volumes.
|
|---|
| Preservation: |
• Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
• Protect from light.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group II
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, solid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
Information regarding the types of filters used for collection of aerosol samples to be analyzed for toxins is limited. Sample collectors also should consult the Sample Collection Plan (SCP), Health and Safety Plan (HASP) and/or analytical laboratory to determine the appropriate air collection filter(s).
|
|---|
| Method(s) Listed in SAM 2017 |
Confirmatory:
• [LC-UV] Adapted from Journal of Pharmaceutical and Biomedical Analysis (1989) 7(3): 369-375 [Tier II]
|
|---|
| Analyte: |
Picrotoxin
|
|---|
| Sample Type: |
Particulate (swabs, wipes, dust socks)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container
|
|---|
| Sample Container Size: |
At least 2 sterile, synthetic, and moistened wipes, swabs, or dust socks
|
|---|
| Preservation: |
• Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
• Protect from light.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group II
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, solid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Confirmatory:
• [LC-UV] Adapted from Journal of Pharmaceutical and Biomedical Analysis (1989) 7(3): 369-375 [Tier II]
|
|---|
| Analyte: |
Ricin and Ricinine (ricin marker)
|
|---|
| Sample Type: |
Aerosol (filter/cassette, liquid impinger)
|
|---|
| Sampling Device/Medium: |
Filter: Sterile MCE or PTFE filter. Place the filter in a sterile leak proof container.
Impinger: Transfer contents into sterile leak proof container for shipping.
|
|---|
| Sample Container Size: |
Volumes of collected air samples are dependent on the concentration of the toxin and the collection device used. Sample collectors should consult the incident-specific Sample Collection Plan (SCP), Health and Safety Plan (HASP) and/or analytical laboratory for appropriate volumes.
|
|---|
| Preservation: |
• Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
• Avoid freeze-thaw cycles during transport of samples collected for ricin.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group III
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, liquid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
Information regarding the types of filters used for collection of aerosol samples to be analyzed for toxins is limited. Sample collectors also should consult the Sample Collection Plan (SCP), Health and Safety Plan (HASP) and/or analytical laboratory to determine the appropriate air collection filter(s).
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [LFA] Adapted from Biosecurity and Bioterrorism: Biodefense Strategy, Practice, and Science (2013)
11(4): 237-250 [Tier I]
• [ELISA] Adapted from Journal of Food Protection (2005) 68(6): 1294-1301 [Tier II]
• [ECL] Adapted from Journal of AOAC International (2008) 91(2): 376-382 [Tier II]
• as Ricinine [LC-MS-MS] EPA 600/R-13/022 (EPA/CDC) [Tier I / Tier II]
Confirmatory and Biological Activity:
• [Isotope Dilution LC-MS / MALDI-TOF MS] Adapted from Analytical Chemistry (2011) 83: 2897-2905 [Tier II]
|
|---|
| Analyte: |
Ricin and Ricinine (ricin marker)
|
|---|
| Sample Type: |
Water (surface water, waste water, drinking water)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container
|
|---|
| Sample Container Size: |
100 mL
|
|---|
| Preservation: |
• Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
• Avoid freeze-thaw cycles during transport of samples collected for ricin.
NOTE: For treated water, add 50 mg sodium thiosulfate / 1-L sample
NOTE: For samples collected for detection and measurement of ricinine in treated water, add the following to the sample container prior to collection:
• 8 mg sodium thiosulfate/100mL sample
• 6.4 mg sodium omadine / 100-mL sample
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group III
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, liquid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [LFA] Adapted from Biosecurity and Bioterrorism: Biodefense Strategy, Practice, and Science (2013)
11(4): 237-250 [Tier I]
• [ELISA] Adapted from Journal of Food Protection (2005) 68(6): 1294-1301
[Tier II]
• [ECL] Adapted from Journal of AOAC International (2008)
91(2): 376-382 [Tier II]
• as Ricinine [LC-MS-MS] EPA 600/R-13/022
(EPA/CDC)
[Tier I / Tier II]
Confirmatory and Biological Activity:
• [Isotope Dilution LC-MS / MALDI-TOF MS] Adapted from Analytical Chemistry (2011) 83: 2897-2905
[Tier II]
|
|---|
| Analyte: |
Ricin and Ricinine (ricin marker)
|
|---|
| Sample Type: |
Solid (soil, powder)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container
|
|---|
| Sample Container Size: |
50 - 100 mL
|
|---|
| Preservation: |
• Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
• Avoid freeze-thaw cycles during transport of samples collected for ricin.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group III
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, liquid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [LFA] Adapted from Biosecurity and Bioterrorism: Biodefense Strategy, Practice, and Science (2013)
11(4): 237-250 [Tier I]
• [ELISA] Adapted from Journal of Food Protection (2005) 68(6): 1294-1301
[Tier II]
• [ECL] Adapted from Journal of AOAC International (2008)
91(2): 376-382 [Tier II]
• as Ricinine [LC-MS-MS] EPA 600/R-13/022
(EPA/CDC)
[Tier I / Tier II]
Confirmatory and Biological Activity:
• [Isotope Dilution LC-MS / MALDI-TOF MS] Adapted from Analytical Chemistry (2011) 83: 2897-2905
[Tier II]
|
|---|
| Analyte: |
Ricin and Ricinine (ricin marker)
|
|---|
| Sample Type: |
Particulate (swabs, wipes, dust socks)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container
|
|---|
| Sample Container Size: |
At least 2 sterile, synthetic, and moistened wipes, swabs, or dust socks
|
|---|
| Preservation: |
• Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
• Avoid freeze-thaw cycles during transport of samples collected for ricin.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group III
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, liquid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [LFA] Adapted from Biosecurity and Bioterrorism: Biodefense Strategy, Practice, and Science (2013)
11(4): 237-250 [Tier I]
• [ELISA] Adapted from Journal of Food Protection (2005) 68(6): 1294-1301
[Tier II]
• [ECL] Adapted from Journal of AOAC International (2008)
91(2): 376-382 [Tier II]
• as Ricinine [LC-MS-MS] EPA 600/R-13/022
(EPA/CDC)
[Tier I / Tier II]
Confirmatory and Biological Activity:
• [Isotope Dilution LC-MS / MALDI-TOF MS] Adapted from Analytical Chemistry (2011) 83: 2897-2905
[Tier II]
|
|---|
| Analyte: |
Saxitoxins (STX, NEOSTX, dcSTX, dcNEOSTX, doSTX, GTX 1-6, dcGTX 1-4)
|
|---|
| Sample Type: |
Solid (soil, powder)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container - amber glass or amber PETG (or protect from light)
|
|---|
| Sample Container Size: |
50 - 100 mL
|
|---|
| Preservation: |
Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group I
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, liquid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [Receptor binding assay] Method 2011.27 (AOAC) [Tier II]
• [ELISA] Adapted from Toxicon (2009) 54: 313-320 [Tier II]
Confirmatory:
• [HILIC MS-MS] Adapted from Journal of Chromatography A (2015) 1387: 1-12 [Tier II]
|
|---|
| Analyte: |
Saxitoxins (STX, NEOSTX, dcSTX, dcNEOSTX, doSTX, GTX 1-6, dcGTX 1-4)
|
|---|
| Sample Type: |
Water (surface water, waste water, drinking water)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container - amber glass or amber PETG (or protect from light)
|
|---|
| Sample Container Size: |
A minimum of 100 mL
|
|---|
| Preservation: |
Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
NOTE: For treated water, add 100 - 1,000 mg/L sodium thiosulfate or 100 mg/L ascorbic acid
NOTE: For untreated freshwater samples to be analyzed using ELISA kits, 10X concentrated diluent is added to the sample container prior to or immediately following sample collection. Therefore, coordinate with receiving laboratory(ies) prior to sample collection.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group I
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, liquid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [Receptor binding assay] Method 2011.27 (AOAC) [Tier II]
• [ELISA] Harmful Algae (2016) 56: 77-90 [Tier I]
Confirmatory:
• [HILIC MS-MS] Adapted from Journal of Chromatography A (2015) 1387: 1-12 [Tier II]
|
|---|
| Analyte: |
Saxitoxins (STX, NEOSTX, dcSTX, dcNEOSTX, doSTX, GTX 1-6, dcGTX 1-4)
|
|---|
| Sample Type: |
Particulate (swabs, wipes, dust socks)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container - amber glass or amber PETG (or protect from light)
|
|---|
| Sample Container Size: |
At least 2 sterile, synthetic, and moistened wipes or swabs, or dust socks
|
|---|
| Preservation: |
Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group I
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, liquid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [Receptor binding assay] Method 2011.27 (AOAC) [Tier II]
• [ELISA] Adapted from Toxicon (2009) 54: 313-320 [Tier II]
Confirmatory:
• [HILIC MS-MS] Adapted from Journal of Chromatography A (2015) 1387: 1-12 [Tier II]
|
|---|
| Analyte: |
Saxitoxins (STX, NEOSTX, dcSTX, dcNEOSTX, doSTX, GTX 1-6, dcGTX 1-4)
|
|---|
| Sample Type: |
Aerosol (filter/cassette, liquid impinger)
|
|---|
| Sampling Device/Medium: |
Filter: Sterile MCE or PTFE filter. Place the filter in a sterile leak proof container.
Impinger: Transfer contents into sterile leak proof container for shipping.
NOTE: Use amber glass or amber PETG containers (or protect from light)
|
|---|
| Sample Container Size: |
Volumes of collected air samples are dependent on the concentration of the toxin and the collection device used. Sample collectors should consult the incident-specific Sample Collection Plan (SCP), Health and Safety Plan (HASP) and/or analytical laboratory for appropriate volumes.
|
|---|
| Preservation: |
Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group I
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, liquid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
Information regarding the types of filters used for collection of aerosol samples to be analyzed for toxins is limited. Sample collectors also should consult the Sample Collection Plan (SCP), Health and Safety Plan (HASP) and/or analytical laboratory to determine the appropriate air collection filter(s).
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [Receptor binding assay] Method 2011.27 (AOAC) [Tier II]
• [ELISA] Adapted from Toxicon (2009) 54: 313-320 [Tier II]
Confirmatory:
• [HILIC MS-MS] Adapted from Journal of Chromatography A (2015) 1387: 1-12 [Tier II]
|
|---|
| Analyte: |
Shiga and Shiga-like Toxins
|
|---|
| Sample Type: |
Particulate (swabs, wipes, dust socks)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container
|
|---|
| Sample Container Size: |
At least 2 sterile, synthetic, and moistened wipes or swabs, or dust socks
|
|---|
| Preservation: |
• Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
• Avoid freeze-thaw cycles during transport.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution. .
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group I
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, liquid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [ELISA] Adapted from Austin Immunology (2016) 1(2): 1007: 1-7 [Tier I / Tier II]
Confirmatory:
• [LC-MS-MS] Adapted from Analytical Chemistry (2014) 86: 4698-4706 [Tier II]
|
|---|
| Analyte: |
Shiga and Shiga-like Toxins
|
|---|
| Sample Type: |
Water (surface water, waste water, drinking water)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container
|
|---|
| Sample Container Size: |
100 mL
|
|---|
| Preservation: |
• Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
• Avoid freeze-thaw cycles during transport.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group I
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, liquid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [ELISA] Adapted from Austin Immunology (2016) 1(2): 1007: 1-7 [Tier I / Tier II]
Confirmatory:
• [LC-MS-MS] Adapted from Analytical Chemistry (2014) 86: 4698-4706 [Tier II]
|
|---|
| Analyte: |
Shiga and Shiga-like Toxins
|
|---|
| Sample Type: |
Solid (soil, powder)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container
|
|---|
| Sample Container Size: |
50 - 100 mL
|
|---|
| Preservation: |
• Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
• Avoid freeze-thaw cycles during transport.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution. .
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group I
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, liquid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [ELISA] Adapted from Austin Immunology (2016) 1(2): 1007: 1-7 [Tier I / Tier II]
Confirmatory:
• [LC-MS-MS] Adapted from Analytical Chemistry (2014) 86: 4698-4706 [Tier II]
|
|---|
| Analyte: |
Shiga and Shiga-like Toxins
|
|---|
| Sample Type: |
Aerosol (filter/cassette, liquid impinger)
|
|---|
| Sampling Device/Medium: |
Filter: Sterile MCE or PTFE filter. Place the filter in a sterile leak proof container.
Impinger: Transfer contents into sterile leak proof container for shipping.
|
|---|
| Sample Container Size: |
Volumes of collected air samples are dependent on the concentration of the toxin and the collection device used. Sample collectors should consult the incident-specific Sample Collection Plan (SCP), Health and Safety Plan (HASP) and/or analytical laboratory for appropriate volumes.
|
|---|
| Preservation: |
• Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
• Avoid freeze-thaw cycles during transport.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution. .
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group I
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, liquid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
Information regarding the types of filters used for collection of aerosol samples to be analyzed for toxins is limited. Sample collectors also should consult the Sample Collection Plan (SCP), Health and Safety Plan (HASP) and/or analytical laboratory to determine the appropriate air collection filter(s).
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [ELISA] Adapted from Austin Immunology (2016) 1(2): 1007: 1-7 [Tier I / Tier II]
Confirmatory:
• [LC-MS-MS] Adapted from Analytical Chemistry (2014) 86: 4698-4706 [Tier II]
|
|---|
| Analyte: |
Staphylococcal enterotoxins (SEA, SEB, SEC, SED, SEE)
|
|---|
| Sample Type: |
Particulate (swabs, wipes, dust socks)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container
|
|---|
| Sample Container Size: |
At least 2 sterile, synthetic, and moistened wipes or swabs, or dust socks
|
|---|
| Preservation: |
• Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
• Avoid freeze-thaw cycles during transport.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group I
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, solid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [ELFA] 2007.06 (AOAC) [Tier II]
• [ECL] Adapted from Journal of AOAC International (2014) 97(3): 862-867 [Tier III]
Confirmatory:
• [ELISA] Adapted from Letters in Applied Microbiology (2011) 52: 468-474 [Tier II]
|
|---|
| Analyte: |
Staphylococcal enterotoxins (SEA, SEB, SEC, SED, SEE)
|
|---|
| Sample Type: |
Solid (soil, powder)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container
|
|---|
| Sample Container Size: |
50 - 100 mL
|
|---|
| Preservation: |
• Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
• Avoid freeze-thaw cycles during transport.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group I
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, solid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [ELFA] 2007.06 (AOAC) [Tier II]
• [ECL] Adapted from Journal of AOAC International (2014) 97(3): 862-867 [Tier III]
Confirmatory:
• [ELISA] Adapted from Letters in Applied Microbiology (2011) 52: 468-474 [Tier II]
|
|---|
| Analyte: |
Staphylococcal enterotoxins (SEA, SEB, SEC, SED, SEE)
|
|---|
| Sample Type: |
Aerosol (filter/cassette, liquid impinger)
|
|---|
| Sampling Device/Medium: |
Filter: Sterile MCE or PTFE filter. Place the filter in a sterile leak proof container.
Impinger: Transfer contents into sterile leak proof container for shipping.
|
|---|
| Sample Container Size: |
Volumes of collected air samples are dependent on the concentration of the toxin and the collection device used. Sample collectors should consult the incident-specific Sample Collection Plan (SCP), Health and Safety Plan (HASP) and/or analytical laboratory for appropriate volumes.
|
|---|
| Preservation: |
• Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
• Avoid freeze-thaw cycles during transport.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group I
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, solid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
Information regarding the types of filters used for collection of aerosol samples to be analyzed for toxins is limited. Sample collectors also should consult the Sample Collection Plan (SCP), Health and Safety Plan (HASP) and/or analytical laboratory to determine the appropriate air collection filter(s).
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [ELFA] 2007.06 (AOAC) [Tier II]
• [ECL] Adapted from Journal of AOAC International (2014) 97(3): 862-867 [Tier III]
Confirmatory:
• [ELISA] Adapted from Letters in Applied Microbiology (2011) 52: 468-474 [Tier II]
|
|---|
| Analyte: |
Staphylococcal enterotoxins (SEA, SEB, SEC, SED, SEE)
|
|---|
| Sample Type: |
Water (surface water, waste water, drinking water)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container
|
|---|
| Sample Container Size: |
100 mL
|
|---|
| Preservation: |
• Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
• Avoid freeze-thaw cycles during transport.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group I
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, solid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [ELFA] 2007.06 (AOAC) [Tier II]
• [ECL] Adapted from Journal of AOAC International (2014) 97(3): 862-867 [Tier III]
Confirmatory:
• [ELISA] Adapted from Letters in Applied Microbiology (2011) 52: 468-474 [Tier II]
|
|---|
| Analyte: |
T-2 Mycotoxin (T-2, HT-2)
|
|---|
| Sample Type: |
Aerosol (filter/cassette, liquid impinger)
|
|---|
| Sampling Device/Medium: |
Filter: Sterile MCE or PTFE filter. Place the filter in a sterile leak proof container.
Impinger: Transfer contents into sterile leak proof container for shipping.
|
|---|
| Sample Container Size: |
Volumes of collected air samples are dependent on the concentration of the toxin and the collection device used. Sample collectors should consult the incident-specific Sample Collection Plan (SCP), Health and Safety Plan (HASP) and/or analytical laboratory for appropriate volumes.
|
|---|
| Preservation: |
Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group I
Aircraft: Cargo only
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, solid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
Information regarding the types of filters used for collection of aerosol samples to be analyzed for toxins is limited. Sample collectors also should consult the Sample Collection Plan (SCP), Health and Safety Plan (HASP) and/or analytical laboratory to determine the appropriate air collection filter(s).
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [ELISA] Adapted from Journal of Food Protection (2005) 68(6): 1294-1301 [Tier II]
Confirmatory:
• [LC-MS] Adapted from Rapid Communications in Mass Spectrometry (2006) 20(9): 1422-1428 [Tier II]
|
|---|
| Analyte: |
T-2 Mycotoxin (T-2, HT-2)
|
|---|
| Sample Type: |
Solid (soil, powder)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container
|
|---|
| Sample Container Size: |
50 - 100 mL
|
|---|
| Preservation: |
Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group I
Aircraft: Cargo only
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, solid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [ELISA] Adapted from Journal of Food Protection (2005) 68(6): 1294-1301 [Tier II]
Confirmatory:
• [LC-MS] Adapted from Rapid Communications in Mass Spectrometry (2006) 20(9): 1422-1428 [Tier II]
|
|---|
| Analyte: |
T-2 Mycotoxin (T-2, HT-2)
|
|---|
| Sample Type: |
Water (surface water, waste water, drinking water)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container
|
|---|
| Sample Container Size: |
100 mL
|
|---|
| Preservation: |
Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
NOTE: For treated water, add 8 mg sodium thiosulfate and 6.4 mg sodium omadine / 100-mL sample
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group I
Aircraft: Cargo only
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, solid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [ELISA] Adapted from Journal of Food Protection (2005) 68(6): 1294-1301 [Tier II]
Confirmatory:
• [LC-MS] Adapted from Rapid Communications in Mass Spectrometry (2006) 20(9): 1422-1428 [Tier II]
|
|---|
| Analyte: |
T-2 Mycotoxin (T-2, HT-2)
|
|---|
| Sample Type: |
Particulate (swabs, wipes, dust socks)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container
|
|---|
| Sample Container Size: |
At least 2 sterile, synthetic, and moistened wipes or swabs, or dust socks
|
|---|
| Preservation: |
Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group I
Aircraft: Cargo only
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, solid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [ELISA] Adapted from Journal of Food Protection (2005) 68(6): 1294-1301 [Tier II]
Confirmatory:
• [LC-MS] Adapted from Rapid Communications in Mass Spectrometry (2006) 20(9): 1422-1428 [Tier II]
|
|---|
| Analyte: |
Tetrodotoxin
|
|---|
| Sample Type: |
Particulate (swabs, wipes, dust socks)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container
|
|---|
| Sample Container Size: |
At least 2 sterile, synthetic, and moistened wipes or swabs, or dust socks
|
|---|
| Preservation: |
Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group I
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, solid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [Receptor binding assay] Method 2011.27 (AOAC) [Tier II]
Confirmatory:
• [LC-MS-MS] Adapted from Toxicon (2016) 119: 64-71 [Tier II]
|
|---|
| Analyte: |
Tetrodotoxin
|
|---|
| Sample Type: |
Aerosol (filter/cassette, liquid impinger)
|
|---|
| Sampling Device/Medium: |
Filter: Sterile MCE or PTFE filter. Place the filter in a sterile leak proof container.
Impinger: Transfer contents into sterile leak proof container for shipping.
|
|---|
| Sample Container Size: |
Volumes of collected air samples are dependent on the concentration of the toxin and the collection device used. Sample collectors should consult the incident-specific Sample Collection Plan (SCP), Health and Safety Plan (HASP) and/or analytical laboratory for appropriate volumes.
|
|---|
| Preservation: |
Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group I
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, solid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
Information regarding the types of filters used for collection of aerosol samples to be analyzed for toxins is limited. Sample collectors also should consult the Sample Collection Plan (SCP), Health and Safety Plan (HASP) and/or analytical laboratory to determine the appropriate air collection filter(s).
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [Receptor binding assay] Method 2011.27 (AOAC) [Tier II]
Confirmatory:
• [LC-MS-MS] Adapted from Toxicon (2016) 119: 64-71 [Tier II]
|
|---|
| Analyte: |
Tetrodotoxin
|
|---|
| Sample Type: |
Solid (soil, powder)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container
|
|---|
| Sample Container Size: |
50 - 100 mL
|
|---|
| Preservation: |
Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group I
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, solid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [Receptor binding assay] Method 2011.27 (AOAC) [Tier II]
Confirmatory:
• [LC-MS-MS] Adapted from Toxicon (2016) 119: 64-71 [Tier II]
|
|---|
| Analyte: |
Tetrodotoxin
|
|---|
| Sample Type: |
Water (surface water, waste water, drinking water)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container
|
|---|
| Sample Container Size: |
100 mL
|
|---|
| Preservation: |
Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group I
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, solid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [Receptor binding assay] Method 2011.27 (AOAC) [Tier II]
Confirmatory:
• [LC-MS-MS] Adapted from Toxicon (2016) 119: 64-71 [Tier II]
|
|---|
| Analyte: |
α-Amanitin
|
|---|
| Sample Type: |
Aerosol (filter/cassette, liquid impinger)
|
|---|
| Sampling Device/Medium: |
Filter: Sterile MCE or PTFE filter. Place the filter in a sterile leak proof container.
Impinger: Transfer contents into sterile leak proof container for shipping.
|
|---|
| Sample Container Size: |
Volumes of collected air samples are dependent on the concentration of the toxin and the collection device used. Sample collectors should consult the incident-specific Sample Collection Plan (SCP), Health and Safety Plan (HASP) and/or analytical laboratory for appropriate volumes.
|
|---|
| Preservation: |
Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group II
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, solid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
Information regarding the types of filters used for collection of aerosol samples to be analyzed for toxins is limited. Sample collectors also should consult the Sample Collection Plan (SCP), Health and Safety Plan (HASP) and/or analytical laboratory to determine the appropriate air collection filter(s).
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [ELISA] Adapted from Journal of Food Protection (2005) 68(6): 1294-1301 [Tier II]
Confirmatory:
• [LC-MS-MS] EPA 600/R-13/022 (EPA) [Tier II]
|
|---|
| Analyte: |
α-Amanitin
|
|---|
| Sample Type: |
Particulate (swabs, wipes, dust socks)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container
|
|---|
| Sample Container Size: |
At least 2 sterile, synthetic, and moistened wipes or swabs, or dust socks
|
|---|
| Preservation: |
Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group II
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, solid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [ELISA] Adapted from Journal of Food Protection (2005) 68(6): 1294-1301 [Tier II]
Confirmatory:
• [LC-MS-MS] EPA 600/R-13/022 (EPA) [Tier II]
|
|---|
| Analyte: |
α-Amanitin
|
|---|
| Sample Type: |
Water (surface water, waste water, drinking water)
|
|---|
| Sampling Device/Medium: |
Polypropylene container with polyethylene screw-cap
|
|---|
| Sample Container Size: |
100 mL
|
|---|
| Preservation: |
• Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
NOTE: For treated water:
• Add 8 mg sodium thiosulfate and 6.4 mg sodium omadine / 100-mL sample.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
NOTE: 28 days for water samples
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group II
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, solid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
|
|---|
| Notes: |
N/A
|
|---|
| Method(s) Listed in SAM 2017 |
Presumptive:
• [ELISA] Adapted from Journal of Food Protection (2005) 68(6): 1294-1301 [Tier II]
Confirmatory:
• [LC-MS-MS] EPA 600/R-13/022 (EPA) [Tier II]
|
|---|
| Analyte: |
α-Amanitin
|
|---|
| Sample Type: |
Solid (soil, powder)
|
|---|
| Sampling Device/Medium: |
Sterile leak proof container
|
|---|
| Sample Container Size: |
50 - 100 g
|
|---|
| Preservation: |
Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature ≤ 10°C.
|
|---|
| Holding Time: |
Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory.
|
|---|
| Packaging: |
Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.
Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (see shipping label information). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73 (http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels.
Belongs to Packing Group II
|
|---|
| Shipping Label: |
Environmental samples: The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling.
For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, solid, n.o.s." AND
• UN# 2811 (inhalation hazard),
• UN# 3172 (liquid hazard), or
• UN# 3462 (solid hazard)
NOTE: For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient.
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| Notes: |
N/A
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| Method(s) Listed in SAM 2017 |
Presumptive:
• [ELISA] Adapted from Journal of Food Protection (2005) 68(6): 1294-1301 [Tier II]
Confirmatory:
• [LC-MS-MS] EPA 600/R-13/022 (EPA) [Tier II]
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