Topic: Continuing Development of Alternative High-Throughput Screens to Determine Endocrine Disruption, Focusing on Androgen Receptor, Steroidogenesis, and Thyroid Pathways

The Federal Insecticide, Fungicide, and Rodenticide Act Scientific Advisory Panel (SAP) met on November 28-29, 2017 to consider and review Alternative High-Throughput Screens to Determine Endocrine Disruption, Focusing on Androgen Receptor, Steroidogenesis, and Thyroid Pathways.

Links to meeting materials for this SAP meeting are available below. These materials are published in Docket EPA-HQ-OPP-2017-0214. 

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• Transmittal Memo for Primary Materials (White Paper and Charge) (PDF)(1 pp, 2.0 M)
  • Charge to the Panel (November 28-30, 2017) (PDF)(4 pp, 131 K)
  • White Paper (November 28-30, 2017) (PDF)(159 pp, 3 M)
• Transmittal Memo of Steroidogenisis Background Materials (PDF)(2 pp, 3.7 M)
  • Supplemental File 1: Normalized MTT Cell Viability Data (Multi-Concentration Response level 3 data from invitrodb_v3) for all 654 Chemicals (XLS)(2.0 M)
  • Supplemental File 2: Plots of MTT Cell Viability Data (With Multi-Concentration Response Level 6 Data From invitrodb_v3) for all 654 Chemical (PDF)(672 pp, 7.7 M)
  • Supplemental File 3: Master Data Table of Steroid Hormone Data Used in the Analyses (XLS)(9.5 M)
  • Supplemental File 4: P-value Results of ANOVA for 654 Chemicals (TXT)(1.2 M)
  • Supplemental File 5: Binary Logic Describing the Positive or Negative Result, Based on the OECD TG 456 Criteria for Positives, for Each Chemical-Hormone Pair (TXT) (64 K)
  • Supplemental File 6: Binary String Logic Used to Implement the OECD TG 456-Based Analysis for Positive Responses (TXT)(210 K)
  • Supplemental File 7: Steroid Hormone Response Plots and Mean Mahalanobis Distance Plots for 654 Chemicals With Multi-Concentration Response Data (PDF)(766 pp, 4.6 M)
  • Supplemental File 8: Radar plots by Chemical for the 11 Steroid Hormone Analytes (PDF)(766 pp, 7.9 M)
  • Supplemental File 9: Maximum Mean Mahalanobis Distance (maxmMd) by Chemical (TXT)(86 K)
  • Supplemental File 10: Comparison of the OECD Inter-Laboratory Validation Results and HT H295R for E2 and T Synthesis (PDF)(6 pp, 507 K)
  • Supplemental File 11: OECD Reference Chemical Performance in HT H295R Versus OECD Inter-Laboratory Results and Literature-Reported Results (PDF)(7 pp, 464 K)
  • Supplemental File 12: Evaluation of the Reproducibility of the maxmMd Metric Across Replicate Experimental Blocks for 107 Chemicals (PDF)(2 pp, 514 K)
  • Supplemental File 13: R scripts used to create the analyses in this chapter as a PDF (PDF)(4 pp, 32 M)
• Transmittal Memo for Androgen Receptor Background Material (PDF)(1 pp, 1.8 M)
  • Supplemental File 1. AR Assay Annotation (PDF)(67 pp, 915 K)

• Panel Members (November 28-29, 2017) (PDF)(4 pp, 74 K)
• Charge to the Panel (November 28-29, 2017) (PDF)(4 pp, 131 K)
• White Paper (November 28-30, 2017) (PDF)(159 pp, 3 MB)
• Biosketches of Science Review Board (PDF)(4 pp, 32 K)
• Transcript (November 28-29, 2017) (PDF)(498 pp, 2 MB)
• Meeting Minutes/Final Report (November 28-29, 2017) (PDF)(69 pp, 815 K)