If the entire pellet will be analyzed and examined, simply divide the measured volume by the number of subsamples to be processed and transfer this calculated volume to each Leighton tube.
Calculate the actual volume examined:Volume transferred/Total resuspended concentrate x Volume filtered
10 L filtered 0.4 mL packed pellet 1 IMS reaction | 10 L filtered 1.8 mL packed pellet 4 IMS reactions | |
---|---|---|
Estimated Resuspended Volume | 5 mL | 1.8 mL/0.5 mL x 5 mL = 18 mL |
Measured Total Resuspended Concentrate | 5.6 mL | 19 mL |
Number of Subsamples Processed | 1 | 4 |
Volume Transferred to each Leighton Tube | 5.6 mL | 4.75 mL |
Total Volume Transferred | 5.6 mL | 19 mL |
Volume Examined | 5.6 mL/5.6 mL x 10 L = 10 L | 19 ml/19 ml x 10 L = 10 L |
Alternatively, add reagent water to total resuspended concentrate volume to equal a number divisible by 5 and always transfer 5 mL to each Leighton tube. For example, add 1 mL to the second example above to reach 20 mL and transfer 5 mL for each IMS reaction.
Select the link for an example, if only a portion of the pellet will be analyzed and examined.