Method 1622/1623
Immunomagnetic Separation
Transfer Volume Calculations
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If the entire pellet will be analyzed and examined, simply divide the measured volume by the number of subsamples to be processed and transfer this calculated volume to each Leighton tube.

Calculate the actual volume examined:Volume transferred/Total resuspended concentrate x Volume filtered

10 L filtered
0.4 mL packed pellet
1 IMS reaction
10 L filtered
1.8 mL packed pellet
4 IMS reactions
Estimated Resuspended Volume5 mL1.8 mL/0.5 mL x 5 mL = 18 mL
Measured Total Resuspended Concentrate5.6 mL19 mL
Number of Subsamples Processed14
Volume Transferred to each Leighton Tube5.6 mL4.75 mL
Total Volume Transferred5.6 mL19 mL
Volume Examined5.6 mL/5.6 mL x 10 L = 10 L19 ml/19 ml x 10 L = 10 L

Alternatively, add reagent water to total resuspended concentrate volume to equal a number divisible by 5 and always transfer 5 mL to each Leighton tube. For example, add 1 mL to the second example above to reach 20 mL and transfer 5 mL for each IMS reaction.

Select the link for an example, if only a portion of the pellet will be analyzed and examined.