Method 1622/1623
Immunomagnetic Separation
Subsample Calculations (1 of 2)
12 of 65
Previous
Show Text
Print Lesson
Submit DR
View DR
Edit Me

Decide on how many subsamples or IMS reactions will be processed based on the pellet size. The table shows the number of subsamples or IMS reactions to be processed for a range of packed pellet sizes based on the 0.5 mL maximum for the Invitrogen IMS kit. The information below the table summarizes important points about processing subsamples. Remember, for LT2 samples, 2 mL of packed pellet or 10 L of equivalent samples volume must be analyzed.

Packed Pellet Size Number of Subsamples (IMS Reactions)
≤ 0.5 mL1
0.5 mL < Pellet ≤ 1 mL2
1 mL < Pellet ≤ 1.5 mL3
1.5 mL < Pellet ≤ 2 mL4
2 mL≥4

Pellet ≤0.5 mL Pellet >0.5 mL
  • Processed in one Leighton tube
  • No calculations are necessary
  • Entire sample is analyzed and examined
  • Split into multiple Leighton tubes each with ≤0.5 mL packed pellet
  • Each Leighton tube is a subsample or an IMS reaction
  • Calculations required
  • Entire sample may or may not be analyzed and examined