Special techniques to increase recoveries from samples with various interferences include:
- Removal of interfering magnetic minerals prior to addition of beads
- Adjustment of the pH of acidic or alkaline samples to 7.0
- Removal of excess debris by rinsing the flat-sided tube
- Removal of excess debris trapped in the bead pellet by washing the pellet in the microcentrifuge tube
- Use of heat dissociation instead of acid dissociation
- Distribution of sample particulates over larger surface area on microscope slide
Detailed protocols for each technique and required quality control (QC) are available in the IMS Supplement.
Tips and Tricks