The purpose of the Leighton tube wash is to remove excess debris from the tube without disturbing the bead pellet. Use this rinse with difficult matrices that exhibit visible debris left in the Leighton tube after aspiration of the remaining supernatant that collects at the bottom of the tube after decanting (Section 13.3.2.11). This wash will remove debris that may interfere with the transfer of the beads to the microcentrifuge tube.