
Mix the water sample concentrate with SL-Buffer A, SL-Buffer B, anti-Cryptosporidium and anti-Giardia beads for 1 hour at ~18 rpm using the rotating mixer.
- Buffers, beads and samples should be at room temperature before use
- SL-buffer A often becomes crystallized during storage in the refrigerator; dissolve by mixing, hand warming, and/or brief warming at 37°C
- Thoroughly resuspend the beads; visually inspect before adding to Leighton tube
Adjust the volume in the Leighton tube to 10 mL, if needed, before adding buffer or beads. Add reagent water if volume is <10 mL. If the volume is >10 mL, adjust the volume of buffers proportionately. There is no need to adjust the bead volume.
It is beneficial to add the IMS buffers after the sample has been transferred instead of prior to transferring the sample to ensure the correct volume of sample and to provide immediate mixing of the sample and buffers.