Remember: the total volume transferred to the Leighton tube should equal 10 mL, which includes the sample water concentrate and reagent water rinses. The 2 mL of IMS buffer is added to the 10 mL of sample.
Rinse the centrifuge tube twice if the entire pellet is transferred. The total rinse volume is divided into 2 separate rinses. Divide the total rinse volume into two rinses. They do not need to be equal. The table shows potential volume scenarios and rinse volumes.
10 mL – sample volume transferred = total rinse volume
Sample Volume Transferred (mL) | Total Rinse Volume |
---|---|
4.2 | 5.8 |
5 | 5 |
5.5 | 4.5 |
6.25 | 3.75 |
No rinses are performed if only a portion of the pellet is transferred, but do add enough reagent water to equal 10mL transferred.
Select the video on the right to view the procedure.