Method 1622/1623
Immunomagnetic Separation
Knowledge Review
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Which of the following statements are correct?
(Select all that apply. More than one statement may be correct.)


A. After decanting the supernatant from the flat-sided tube you can use a pipette to remove additional supernatant from the bottom of the tube.
B. Both the MPC®-6 and MPC®-1 are rocked through 180° for 2 minutes.
C. After adding buffer to the decanted flat-sided tube, you should vortex vigorously to resuspend the pellet.
D. Rinse the flat-sided tube twice with additional buffer to transfer to the microcentrifuge tube.
E. When the MPC®-S magnet is in the vertical position, the pellet forms high on the back wall of the tube.
F. Automatic rocking plates are useful to provide better consistency in rocking the flat-sided tubes.
G. The magnetic particle concentrator is used to attract the bead-organism complexes to the magnet.