Method 1622/1623
Immunomagnetic Separation
Adjusting pH
33 of 46
Previous
Pause
Play
Replay
IMS Supplement
Show Text
Print Lesson
Submit DR
View DR

Some source water samples may produce a pellet with non-neutral pH characteristics. Low recoveries could result if acidity or alkalinity of the pellet is not adequately buffered during the IMS process.

Some laboratories have determined the addition of HCl or NaOH to the sample, after buffers have been added, can neutralize the pH to improve recoveries. The pH of the sample can also be checked before buffers are added, and after the 1-hour rotation to ensure the pH is stable.

See the IMS Supplement for the protocol and required quality control.

Buffers, reagents and pH meter

You need the latest free Flash Player Plugin to view this page.