- Add 0.1N HCl so that the pH decreases
- Vortex vigorously; bead-organism complex dissociates and low pH prevents complexes from reforming
- Apply the magnet to the sample to pull beads out of suspension
- Transfer the dissociated sample to a slide containing 1.0N NaOH to neutralize the acid
Repeat to insure that all bead-organism complexes have been dissociated and all organisms have been transferred to slide.