Method 1622/1623
Immunomagnetic Separation
Aspiration
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Aspirate the supernatant of the concentrated water sample to the total resuspended concentrate volume already determined. Do not aspirate below 5 mL for samples with pellets ≤0.5 mL to eliminate potential loss of organisms. Perform this carefully and consistently . The techniques listed below apply to aspiration in a 50-mL, 250-mL, or 500-mL centrifuge tube/bottle.

Reducing the vacuum pressure when aspirating below 30 mL may reduce loss of organisms.

View the videos demonstrating correct and incorrect aspiration; listen to the difference.

2 videos
epa_01.mov 10:30;00 to 10:40;00 - Incorrect Aspiration
epa_01.mov 13:51;00 to 14:00;00 - Correct Aspiration