Method 1622/1623
Immunomagnetic Separation
Beads, Buffers and Equipment
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Reagents, unmixed beads, mixing beads, mixed beads

Mix the water sample concentrate with SL-Buffer A, SL-Buffer B, anti-Cryptosporidium and anti-Giardia beads for 1 hour at ~18 rpm using the rotating mixer.

Adjust the volume in the Leighton tube to 10 mL, if needed, before adding buffer or beads. Add reagent water if volume is <10 mL. If the volume is >10 mL, adjust the volume of buffers proportionately. There is no need to adjust the bead volume.

It is beneficial to add the IMS buffers after the sample has been transferred instead of prior to transferring the sample to ensure the correct volume of sample and to provide immediate mixing of the sample and buffers.