Method 1622/1623
Immunomagnetic Separation
Leighton Tube Wash Introduction (1 of 2)
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The purpose of any additional wash is to remove excess debris from the sample. Excess debris may interfere with staining and/or microscopic examination of the sample and adversely affect organism recoveries.

The need for additional washes may be based on:

Most samples with a pellet ≥0.5 mL will leave some debris in the Leighton tube and bead pellet.