Remove the flat-sided tube from the magnet and quantitatively transfer the bead pellet to the microcentrifuge tube as described here:
- Pipette 0.5 mL of 1X SL-Buffer A onto the flat-side of the tube to gently wash the bead pellet to the bottom of the tube.
- Resuspend the bead pellet by gently sliding the suspension across the length of the flat-side.
- Draw-up the resuspended bead pellet and dispense into a labeled microcentrifuge tube.
- This microcentrifuge tube should be in the MPC®-S with magnet in vertical position as shown on the next screen.
- Rinse the flat-side of the tube twice using 0.5 mL of 1X SL-Buffer A.
- Pipette each rinse into the microcentrifuge tube.
- Allow the flat-sided tube to sit for at least 1 minute to allow any remaining liquid to drain to the bottom, then transfer to the microcentrifuge tube.
Tips and Tricks
Select the video to view a demonstration of resuspension of the bead pellet.