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- Allow buffers and samples to reach room temperature.
- SL-buffer A often becomes crystallized during storage in the refrigerator; dissolve by mixing, hand warming, and/or brief warming at 37°C.
- Add 1 mL of 10X SL Buffer A and 1 mL of 10X SL Buffer B to each flat-sided tube.
- Pre-rinse a pipette with elution buffer.
- Draw up 5 mL of the well-mixed pellet suspension into the pipette and dispense into the labeled flat-sided tube.
- If the entire pellet suspension has been transferred, rinse the centrifuge tube twice with ≤2.5 mL of reagent water per rinse.
- Adjust the volume in the flat-sided tube to 12 mL by adding reagent water.
Select the videos to view demonstrations.