Tips and Tricks
The graduations on the centrifuge tubes are not always accurate, try the following to ensure accuracy:
Use the transfer pipette to measure the actual volume transferred and change rinse volumes as needed.
Or
Use a set of centrifuge tubes calibrated to the resuspended concentrate volumes used.
Plastic, disposable, serological pipettes work well for transfers.
Be sure that the pipette fits into the centrifuge tube used, in both diameter and length.
Any type of pipette aid works; be cognizant of repetitive motion stress when choosing a pipette.