
If recoveries of the IMS control are <70% or variable, check the following:
- Resuspension of the pellet
- Use of the proper quantities of beads, buffers and sample
- Use of a protocol to guarantee the addition of all buffers and beads to all samples
- Rotation speed and time
- Thorough rinses of the centrifuge tube
- Proper bead pellet formation in the Leighton tube
- Proper use of the MPC®-6/MPC®-1
- Thorough resuspension of the bead pellet and rinses in the Leighton tube
- Proper bead pellet formation in the microcentrifuge tube
- Complete dissociation with vigorous vortex
- HCl and NaOH concentrations