Method 1622/1623
Immunomagnetic Separation
Rinsing Centrifuge Tubes
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Remember: the total volume transferred to the Leighton tube should equal 10 mL, which includes the sample water concentrate and reagent water rinses. The 2 mL of IMS buffer is added to the 10 mL of sample.

Rinse the centrifuge tube twice if the entire pellet is transferred. The total rinse volume is divided into 2 separate rinses. Divide the total rinse volume into two rinses. They do not need to be equal. The table shows potential volume scenarios and rinse volumes.

10 mL – sample volume transferred = total rinse volume

Sample Volume Transferred (mL)Total Rinse Volume
4.25.8
55
5.54.5
6.253.75

No rinses are performed if only a portion of the pellet is transferred, but do add enough reagent water to equal 10mL transferred.
Select the video on the right to view the procedure.

Video: 1st choice:EPA_01.mov – 25:29;09 to 28:20;23 with the following sections cut – 26:26;10 to 26:35;25 and 27:39;21 to 28:14;00 2nd choice:Epa_02.mov - 45:34;23-47:41;14