For pellets ≤0.5 mL, quantitatively transfer the entire water sample concentrate to the Leighton tube by following the steps below.
- Pre-rinse a pipette with elution buffer (use 5- or 10-mL pipette)
- Resuspend the packed pellet using vortex, pipette mixing or a combination
- Rinse the sides of the conical portion of the tube while pipette mixing
- Draw up the concentrate into the pipette
- Collect as much of the pellet as possible on the transfer
- Measure and record the volume in the pipette
- Pipette the entire sample into the labeled Leighton tube
- Calculate the volume of reagent water rinses
[10 mL - mL transferred; divide volume between 2 rinses]
Select the video to view the procedure.
For pellets >0.5 mL, follow the steps on the next screen.
1st choice:
Video: epa_01.mov 22:45;20 - 24:53;00
2nd choice:
Epa_01.mov 29:29;04 - 29:59;29 and epa_02.mov 30:00;00 - 35:18;00 with screwups omitted
3rd choice or other possibility - Use pieces of video and fade together -George Method 1693 1 compressed 27:39-27:52; visual check 28:46