Add Hydrochloric acid (HCl) to the microcentrifuge tube, to decrease pH of the sample. Once the complexes are dissociated, the low pH prevents the reformation of bead-organism complexes.
Remember:
- Use commercially available 0.1N HCl
- Purchase in small volumes to use before expiration date
- 50 µL is used for each dissociation
- Aliquot stock 0.1N HCl to microfuge tubes prior to use to facilitate pipetting