Method 1622/1623
Immunomagnetic Separation
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Which of the following statements are correct?
(Select all that apply. More than one statement may be correct.)


A. Pellet standards should be used to compare and estimate the volume of the packed pellet.
B. The pellet is resuspended before transfer to the flat-sided tubes by inverting the centrifuge tube by hand 2 or 3 times.
C. The vortex speed should be reduced on pellet resuspension if excess debris is splattered on the sides of the centrifuge tube.
D. The weight of the centrifuge bottles should be balanced to within 0.5 grams.
E. During aspiration, 10 mL of fluid should be retained for every 0.5 mL of pellet.
F. The pellet sample is aspirated using high vacuum pressure to ensure organisms are pulled out of suspension.
G. Aspiration of the supernatant should be at the air/liquid interface not below the water surface.