Examples: | 0.7 mL packed pellet = 2 subsamples or IMS reactions | 1.8 mL packed pellet = 4 subsamples or IMS reactions |
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Equation | 0.7 mL/0.5 mL x 5 mL = 7 mL |
1.8 mL/0.5 mL x 5 mL = 18 mL |
Total resuspended concentrate: | 7 mL | 18 mL |
Volume of resuspended concentrate transferred to each Leighton tube: | 3.5 mL | 4.5 mL |
Number of subsamples processed: | 2 | 4 |
Volume of resuspended concentrate transferred: | 7 mL | 18 mL |
Or round up to the nearest 5 mL | Alternate calculation | Alternate calculation |
Total resuspended concentrate: | 10 mL | 20 mL |
Volume of resuspended concentrate transferred to each Leighton tube: | 5 mL | 5 ml |
Number of subsamples processed: | 2 | 4 |
Volume of resuspended concentrate transferred: | 10 mL | 20 mL |