Determine the total volume of resuspended concentrate in the centrifuge tube/bottle using the calculation below. You will aspirate to this volume; however, this volume is an estimation, the actual total resuspended volume will be measured. For pellets ≤0.5 mL, aspirate to 5 mL, but not below 5 mL.
This calculation means that every 1 mL of resuspended concentrate is equivalent to 0.1 mL of packed pellet volume; therefore, transferring 3 mL of resuspended concentrate is equivalent to transferring 0.3 mL of packed pellet.Remember, each Leighton tube will contain a maximum of 0.5 mL of packed pellet when using the Invitrogen IMS kit