Observations on DIC that help distinguish an interfering organism from Giardia:
- The wall observed on DIC is not superimposed by the FITC fluorescing wall
- More than one wall
- Uncharacteristically narrow or wide wall
- Variations in wall width
- Openings or breaks in wall
- Internals composed of small spheres
- Internals composed of more than one large distinct mass
- Organism composed of multiple cells
- Presence of mucilage around the organism
- Atypical shape and size
- Presence of colored internal structures
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