After completing this module, the analyst should be able to describe a positive control Giardia cyst and Cryptosporidium oocyst based on FITC and DAPI fluorescence and DIC.
The classic Giardia cyst displays:
- Brilliant apple-green FITC fluorescence on the cyst wall that is crisp and sharp
- Up to four DAPI stained nuclei
- Up to four nuclei, two axonemes and two median bodies visible on DIC
- Correct size and shape; 5-15 µm wide, 8-18 µm long
- No atypical structures or color
The classic Cryptosporidium oocyst displays:
- Brilliant apple-green FITC fluorescence on the oocyst wall that is crisp and sharp
- Up to four DAPI stained nuclei
- Up to four sporozoites visible on DIC
- Correct size and shape; 4-6 µm in diameter
- No atypical structures or color

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