Method 1623
Giardia & Cryptosporidium
Knowledge Review
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Is it Cryptosporidium?

Check the characteristics observed in the photos which support why you think the organism is or is not Cryptosporidium. Once you have decided, check the Yes or No box below. (assume photographs represent the same organism and microscope is adjusted for best contrast and resolution)

1. One sporozoite
2. Three sporozoites
3. No apparent cell wall
4. Atypical internal morphology on DIC
5. Atypical shape
6. Correct shape
7. Atypical fluorescence
8. Brilliant apple-green fluorescence
9. Fluorescence is not crisp and sharp
10. Fluorescence is crisp and sharp
11. More than 4 DAPI stained nuclei
12. Atypical DAPI staining
13. Cell wall on DAPI atypical color
organisms for Knowledge Review
Yes, it is Cryptosporidium.
No, it is not Cryptosporidium.

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