An official website of the United States government.

This is not the current EPA website. To navigate to the current EPA website, please go to This website is historical material reflecting the EPA website as it existed on January 19, 2021. This website is no longer updated and links to external websites and some internal pages may not work. More information »

Draft Test Guidelines for Pesticides and Toxic Substances

The draft guidelines on this page are part of a series of test guidelines that have been developed by EPA for use in the testing of pesticides and toxic substances, and the development of test data for submission to the Agency for review. This is not intended to be a comprehensive compilation of ALL draft guidelines, just those that were issued in draft for public comment.

Although these are draft guidelines that are not yet available in final form, you may consult these guidelines if you are developing a proposed study protocol. However, before you use a draft guideline to generate data that will be submitted to EPA, please check with the appropriate EPA office.

The draft guidelines below are organized by series number and are closed for public comment.

850.1085 - Fish Acute Toxicity Mitigated By Humic Acid
850.1350 - Mysid Chronic Toxicity Test
850.1500 - Fish Life Cycle Toxicity
850.1790 - Chironomid Sediment Toxicity Test
850.1800 - Tadpole/Sediment Subchronic Toxicity Test
850.1850 - Aquatic Food Chain Transfer
850.1900 - Generic Freshwater Microcosm Test, Laboratory
850.1925 - Site-Specific Aquatic Microcosm Test, Laboratory
850.1950 - Field Testing For Aquatic Organisms
870.3500 - Preliminary Developmental Toxicity Screen
870.3600 - Inhalation Developmental Toicity Study
870.5265 - The Salmonella Typhimurium Reverse Mutation Assay
870.8223 - Pharmacokinetic Test
870.8245 - Dermal Pharmacokinetics of DGBE and DGBA
870.8300 - Dermal Absorption for Compounds that are Volatile and Metabolized to Carbon Dioxide
870.8320 - Oral/Dermal Pharmacokinetics
870.8340 - Oral and Inhalation Pharmacokinetic Test
870.8360 - Pharmacokinetics of Isopropanal
870.8380 - Inhalation and Dermal Pharmacokinetics of Commercial Hexane
870.8500 - Toxicokinetic Test
870.8600 - Developmental Neurotoxicity Screen
870.8700 - Subchronic Oral Toxicity Test
870.8800 - Morphologic Transformation of Cells in Culture