The final Health Effects Test Guidelines are generally intended to meet testing requirements for human health impacts of chemical substances under FIFRA and TSCA.

Supplemental Guidance

Test Guidelines/Acute Toxicity - Acute Oral Toxicity Up-And-Down-Procedure
Guidance for Waiving or Bridging of Mammalian Acute Toxicity Tests for Pesticides and Pesticide Products
Guidance for Neurotoxicity Battery, Subchronic Inhalation, Subchronic Dermal and Immunotoxicity Studies
Genetic Toxicology: Integration of in vivo Testing into Standard Repeat Dose Studies
Use of an Alternate Testing Framework for Classification of Eye Irritation Potential of EPA Pesticide Products
Update on the Use of the Local Lymph Node Assay for End Use Pesticide Products and Adoption of the Reduced Dose Protocol for LLNA (rLLNA)

Group A – Acute Toxicity Test Guidelines

870.1000 - Acute Toxicity Testing--Background (December 2002)
870.1100 - Acute Oral Toxicity (December 2002)
870.1200 - Acute Dermal Toxicity (August 1998)
870.1300 - Acute Inhalation Toxicity (August 1998)
870.2400 - Acute Eye Irritation (August 1998)
870.2500 - Acute Dermal Irritation (August 1998)
870.2600 - Skin Sensitization (March 2003)

Group B – Subchronic Toxicity Test Guidelines

870.3050 - Repeated Dose 28-Day Oral Toxicity Study in Rodents (July 2000)
870.3100 - 90-Day Oral Toxicity in Rodents (August 1998)
870.3150 - 90-Day Oral Toxicity in Nonrodents (August 1998)
870.3200 - 21/28-Day Dermal Toxicity (August 1998)
870.3250 - 90-Day Dermal Toxicity (August 1998
870.3465 - 90-Day Inhalation Toxicity (August 1998)
870.3550 - Reproduction/Developmental Toxicity Screening Test (July 2000)
870.3650 - Combined Repeated Dose Toxicity Study with the Reproduction/Developmental Toxicity Screening Test (July 2000)
870.3700 - Prenatal Developmental Toxicity Study (August 1998)
870.3800 - Reproduction and Fertility Effects (August 1998)

Group C – Chronic Toxicity Test Guidelines

870.4100 - Chronic Toxicity (August 1998)
870.4200 - Carcinogenicity (August 1998)
870.4300 - Combined Chronic Toxicity/Carcinogenicity (August 1998)

Group D – Genetic Toxicity Test Guidelines

870.5100 - Bacterial Reverse Mutation Test (August 1998)
870.5140 - Gene Mutation in Aspergillus nidulans (August 1998)
870.5195 - Mouse Biochemical Specific Locus Test (August 1998)
870.5200 - Mouse Visible Specific Locus Test (August 1998)
870.5250 - Gene Mutation in Neurospora crassa (August 1998)
870.5275 - Sex-linked Recessive Lethal Test in Drosophila melanogaster (August 1998)
870.5300 - In vitro Mammalian Cell Gene Mutation Test (August 1998)
870.5375 - In vitro Mammalian Chromosome Aberration Test (August 1998)
870.5380 - Mammalian Spermatogonial Chromosomal Aberration Test (August 1998))
870.5385 - Mammalian Bone Marrow Chromosomal Aberration Test (August 1998)
870.5395 - Mammalian Erythrocyte Micronucleus Test (August 1998)
870.5450 - Rodent Dominant Lethal Assay (August 1998)
870.5460 - Rodent Heritable Translocation Assays (August 1998)
870.5500 - Bacterial DNA Damage or Repair Tests (August 1998)
870.5550 - Unscheduled DNA Synthesis in Mammalian Cells in Culture (August 1998)
870.5575 - Mitotic Gene Conversion in Saccharomyces cerevisiae (August 1998)
870.5900 - In vitro Sister Chromatid Exchange Assay (August 1998)
870.5915 - In vivo Sister Chromatid Exchange Assay (August 1998)

Group E – Neurotoxicity Test Guidelines

870.6100 - Acute and 28-Day Delayed Neurotoxicity of Organophosphorus Substances (August 1998)
870.6200 - Neurotoxicity Screening Battery (August 1998)
870.6300 - Developmental Neurotoxicity Study (August 1998)
870.6500 - Schedule-Controlled Operant Behavior (August 1998)
870.6850 - Peripheral Nerve Function (August 1998)
870.6855 - Neurophysiology Sensory Evoked Potentials (August 1998)

Group F – Special Studies Test Guidelines

870.7200 - Companion Animal Safety (August 1998)
870.7485 - Metabolism and Pharmacokinetics (August 1998)
870.7600 - Dermal Penetration (August 1998)
870.7800 - Immunotoxicity (August 1998)

Group G – Health Effects Chemical-Specific Test Guidelines

870.8355 - Combined Chronic Toxicity/Carcinogenicity Testing of Respirable Fibrous Particles (July 2001)