An official website of the United States government.

This is not the current EPA website. To navigate to the current EPA website, please go to This website is historical material reflecting the EPA website as it existed on January 19, 2021. This website is no longer updated and links to external websites and some internal pages may not work. More information »

Pesticide Registration

E-Submission for Terrestrial and Aquatic Plant Studies

Environmental Fate and Effects Studies

Current as of February 2001

The following general guidance applies to both terrestrial and aquatic plant studies:

  • Data should be provided in files separate from other material such as reviews.
  • Data files should have a special file extension not used for other kinds of files, probably ".dat" (e.g., "ONION.DAT").
  • Missing measurements should be indicated by a dot (".") as in the example below. A missing value code should be required when there are multiple endpoints in the same file.
  • Individual numbers or values on a line are separated by one or more spaces.
  • Lines or rows of data are separated by a hard return
  • Data files should be ASCII.
  • Character data such as variable names or treatment group names should consist of strings of length not more than 8 characters.
  • Ratio endpoints such as emergence (the fraction of seeds that emerge) should be represented as decimal numbers rather than as percentages.

Example of suggested file format

Following is how a file might look for a study with two treatment levels and four measurement endpoints:

10 23

  Height Weight Emergnce Phytotox
C 2.5 3.0 1.00 0.00
C 1.9 2.8 1.00 0.00
1 1.8 2.2 0.88 0.00
1 1.9 2.5 0.79 0.00
2 . . 0.00 .
2 1.0 1.9 0.22 1.00

An explanation of each line follows:

Line 1: Title line
Line 2: Dose values applied to treated groups
Line 3: Names of measurement endpoints (#8 characters per name)
Lines 4 ff: data for individual plots, data columns as follows:

Column 1: Treatment group
Columns 2-5: Values of measurement endpoints. The endpoints are identified on Line 3.
(The 3rd name on Line 3 is the name of the 3rd endpoint from left to right, and so on.)

For further information, please contact Elizabeth Donovan (, 703-347-0227 or Van Vogel (, 703-347-0541.